比较转录组学揭示了新的和差异化的循环RNA反应,这些反应是猪膜巨体中介导干扰素的抗病毒调节的基础
Jiuyi Li1, Oluwaseun Adeyemi1, Laura C Miller2
1Department of Food and Animal Sciences, College of Agriculture, Tennessee State University, Nashville, TN 37209, USA.
Viruses
|October 29, 2025
概括
猪生殖和呼吸系统综合征 (PRRS) 对猪经济产生影响. 这项研究揭示了免疫细胞中循环RNAs (circRNAs) 对抗病毒治疗,特别是干扰素-omega5的反应,这表明了新的治疗点.
科学领域:
- * 病毒学和免疫学
- * 非编码RNA生物学
- * 猪健康 猪健康
背景情况:
- * 猪生殖和呼吸系统综合征 (PRRS) 对猪业构成重大经济挑战.
- *循环RNAs (circRNAs) 正在成为宿主病毒相互作用和免疫反应的关键调节者.
- * 猪膜巨细胞 (PAMs) 是PRRS病毒 (PRRSV) 的首要目标.
研究的目的:
- * 在抗病毒治疗后在PAM中全面描述全基因组的circRNA表达.
- * 调查circRNAs在干扰素介导的抗病毒反应中的作用.
- * 探索差异表达的环RNAs的调节和潜在编码能力.
主要方法:
- *使用RNA测序来分析PAMs中的circRNA表达特征.
- *基因本体学和KEGG通路分析对circRNA相关基因进行.
- * 生物信息工具用于预测miRNA结合位点和评估编码潜力 (ORF,IRES,m6A位点).
主要成果:
- *在使用改性活病毒 (MLV) 疫苗或干扰素亚型 (IFN-α1,IFN-ω5) 治疗后,在PAM中鉴定出超过1000个差异表达的circRNA.
- *circRNA表达特征在治疗组中显示了保留和明显的反应,丰富了免疫和抗病毒途径.
- * 干扰素-omega5 (IFN-ω5) 诱导了特别强的circRNA反应,这表明它在抗病毒防御中起着关键作用.
结论:
- *circRNAs在PAMs对抗病毒刺激的免疫反应中发挥着重要作用,包括PRRSV感染.
- *这项研究突出了一个复杂的circRNA-miRNA调节网络,参与抗病毒防御.
- * 一组circRNAs可能具有非正规编码潜力,为针对PRRSV的抗病毒策略提供新的途径.
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