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压力颗粒核心蛋白在UBP1b酸化体外研究的研究

Anna S Nizkorodova¹, Valeriy Y Kislitsin¹, Andrey V Zhigailov¹

¹M. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty 050012, Kazakhstan.

Plants (Basel, Switzerland)

|October 29, 2025

概括

在植物中,酸化橄氨基酸结合蛋白1B (UBP1B) 可能调节其与mRNA的结合. 这种蛋白质特别结合到5'非翻译区域,抑制了体外蛋白质合成.

科学领域:

分子生物学分子生物学
植物科学植物科学
生物化学生化学

背景情况:

压力颗粒 (SG) 对于细胞应激反应至关重要,但它们在植物中的形成,特别是mRNA招募机制,仍然不清楚.
与SG蛋白的mRNA相互作用的特异性在很大程度上尚未被探索.
橄氨基酸结合蛋白1B (UBP1B) 被认为是植物SG的一个关键组成部分.

研究的目的:

为了研究来自Arabidopsis thaliana的Oligouridylate结合蛋白1B (UBP1B) 的酸化状态.
探索UBP1B酸化在与mRNA相互作用中的作用.
为了确定UBP1B对无细胞系统中蛋白质合成的影响.

主要方法:

在大肠杆菌中表达和净化了Arabidopsis thaliana中的AtUBP1b蛋白.
使用质谱学分析蛋白质酸化和使用放射性标记的体外酸化试验.
使用凝转移试验评估mRNA结合,并在无小麦胚芽细胞系统中评估对蛋白质合成的影响.

主要成果:

质谱检测显示,重组ATUBP1b在氨酸,氨酸和氨酸残留物中被酸化.
在体外酸化试验证实了植物提取物中的AtUBP1b酸化.
凝转移试验表明,AtUBP1b与mRNA的5'-未翻译区域 (5'UTR) 具有特定的结合,从而在无细胞系统中抑制蛋白质合成.

结论:

酸化或脱酸化AtUBP1b可以作为其在植物细胞中的RNA结合活性的调节信号.
AtUBP1b特别与mRNA 5'UTRs结合,这表明它在向的mRNA调节中的作用.
在体外,AtUBP1b抑制蛋白质合成,突出其在压力介导的转化控制中的潜在功能.

关键词:

在UBP1bb上这是一种RNA结合蛋白.凝转移发生在凝上酸化的方法是:光化.压力颗粒是压力颗粒.

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