针对性下一代测序使用支气管支气管洗液样本用于诊断肺部感染:单中心回顾性研究
Jinbao Huang1,2, Ling Ye2, Heng Weng2
1Shengli Clinical Medical College of Fujian Medical University, Fuzhou, China.
Frontiers in microbiology
|October 29, 2025
概括
与传统方法相比,支气管洗液 (BALF) 的目标下一代测序 (tNGS) 为诊断肺部感染 (PI) 提供了更高的灵敏度和准确性. 这种先进的技术显著影响临床治疗计划,使其成为一种有价值的诊断工具.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 传染病 传染病 传染病
- 基因组测序是一种基因组测序.
背景情况:
- 肺部感染 (PI) 是一个重大的诊断挑战.
- 传统的微生物学测试 (CMT) 和培养方法在灵敏度和速度方面存在局限性.
- 目标下一代测序 (tNGS) 提供了潜在的病原体检测进步.
研究的目的:
- 为了评估tNGS的诊断价值,在疑似PIs的患者中使用支气管支气管洗液 (BALF) 样本.
- 将tNGS的诊断效率与传统文化和CMT进行比较.
主要方法:
- 一项回顾性研究,涉及110名疑似PI患者.
- 同时分析使用tNGS,传统培养和CMT的BALF样本.
- 三种方法检测灵敏度,特异性和准确性的比较.
主要成果:
- tNGS在PI诊断方面表现出比培养和CMT显著更高的灵敏度和准确性 (P < 0.001).
- 培养表现出比tNGS和CMT更高的特异性 (P < 0.001).
- 在45.5%的患者中,tNGS结果直接影响了临床治疗计划,强调了其临床实用性.
结论:
- tNGS是PIs的快速和准确的辅助诊断方法,在灵敏度和准确性方面超过传统方法.
- 将tNGS整合到临床实践中可以导致肺部感染的更精确,更有效的管理策略.
- 进一步的研究,可能包括人工智能,可以提高tNGS在诊断PI中的能力和应用.
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