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相关概念视频

Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.6K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.5K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

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Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
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相关实验视频

Updated: Jan 13, 2026

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
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Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

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摄影诱导的超快速双重侵入,使用人工核酸准远程双链DNA.

Zumila Hailili1, Yasuha Watanabe1, Siddhant Sethi1

  • 1Bioscience, Biotechnology and Biomedical Engineering Research Area, Japan Advanced Institute of Science and Technology, Asahidai 1-1, Nomi, Ishikawa, 923-1292, Japan.

Chembiochem : a European journal of chemical biology
|October 29, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种快速的照片诱导双重入侵 (pDI) 方法,用于基因组DNA操纵. 这种新技术使用人工核酸进行快速有效的序列选择性DNA向.

关键词:
在CNVKK中使用.它们是DNA DNA DNA DNA.双重入侵 双重入侵核酸是核酸中的一种.照片交叉链接器 照片交叉链接器

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

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相关实验视频

Last Updated: Jan 13, 2026

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Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

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Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 遗传学 是一个遗传学.

背景情况:

  • 基因组DNA对生物过程至关重要,其异常表达可能导致遗传疾病.
  • 序列选择性DNA识别对于基因操纵至关重要.
  • 使用人工核酸 (CNV,K,C,U) 的照片诱导双重复杂入侵 (pDDI) 已先前开发.

研究的目的:

  • 为了调查pDDI的入侵机制.
  • 开发一种更快,更有效的DNA操纵方法.

主要方法:

  • 研究了pDDI的入侵机制.
  • 开发了一种使用3-cyanovinylcarbazole核oside (K) 的新型照片诱导双重入侵 (pDI) 方法.

主要成果:

  • 发现了pDDI探测器的入侵独立性.
  • 新的pDI方法实现了快速的DNA入侵,只使用K.CNV.
  • 使用pDI方法保持了高的入侵效率.

结论:

  • 快速的pDI方法为特定站点的基因组DNA操纵提供了一个强大的新工具.
  • 这种方法提高了基因工程应用的效率和速度.