红细胞衍生异位体miR-93-5p通过PTEN抑制促进肺癌的进展
Ning Li1, Pushpa Dhilipkannah1, Van K Holden2
1Department of Pathology, University of Maryland School of Medicine, Baltimore, MD, 21021, USA.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|October 29, 2025
概括
红血细胞 (RBC) 通过通过外体细胞通过转移致癌物miR-93-5p来积极促进肺癌. 这一发现揭示了一个新的瘤-红细胞通信轴,为肺癌提供了诊断和治疗途径.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 红细胞 (RBC) 传统上以气体交换和代谢调节而闻名.
- 它们在癌症进展中的作用在很大程度上没有得到认可.
- 微RNAs (miRNAs) 越来越多地涉及癌症的发展和信号传递.
研究的目的:
- 确定红细胞在肺癌进展中的未被认可的作用.
- 研究红细胞和肺癌细胞之间的细胞间通信机制.
- 探索外体 miR-93-5p 在肺癌中的诊断和治疗潜力.
主要方法:
- 从肺癌患者和健康对照患者的红细胞和血中分析miR-93-5p水平.
- 使用细胞培养的功能性测试来研究外体介导的miRNA转移及其对瘤细胞的影响.
- 使用肺癌小鼠模型进行体内研究,以评估红细胞衍生的外体miR-93-5p和治疗干预措施的影响.
- 抑制miR-93-5p和恢复PTEN以评估它们对癌症进展的影响.
主要成果:
- 在肺癌患者的红细胞和红细胞衍生外体中发现了升高的miR-93-5p水平,与晚期和不良预后相关.
- 红细胞衍生异位体miR-93-5p抑制瘤细胞中的PTEN,增强增殖,迁移和入侵.
- 确定了一个双向通信轴,肺癌细胞也将miR-93-5p转移到红细胞.
- 抑制miR-93-5p或PTEN恢复逆转了促进癌症的作用.
- 红细胞衍生的外体miR-93-5p加速了瘤生长,并减少了小鼠模型中的存活率.
- 在小鼠中,miR-93-5p的治疗向抑制了瘤负担,减少了转移,并延长了生存时间.
结论:
- 红细胞在促进肺癌的进展方面发挥着积极的作用,通过瘤原体miR-93-5p的外体转移促进了肺癌的进展.
- 一个新的双向瘤-RBC-瘤通信轴有助于肺癌恶性.
- 红细胞中的外体miR-93-5p代表了肺癌的潜在诊断生物标志物和治疗标.
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