相关实验视频

Updated: Jan 6, 2026

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

在容纳条件下化解精液的化支持成功的体外受精,并改善马的细胞内质精子注射结果

Klaartje Broothaers¹, Daniel Angel-Velez^1,2, Fabiola Le Gaffric Molto¹

¹Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.

|October 29, 2025

概括

在用冷解的精子进行细胞内质精子注射 (ICSI) 或体外受精 (IVF) 之前,使马精子有能力显著提高了胚胎细胞的生产率. 这种新的方法提高了马类胚胎生产效率,而不会影响胚胎积率.

科学领域:

马类生殖生物学马类生殖生物学
辅助生殖技术是一种辅助生殖技术.
胚胎学胚胎学

背景情况:

2022年的一项协议允许使用新鲜精液进行马体体外受精 (IVF).
这一突破使马精子的成功电容化成为可能,为新的应用开辟了道路.

研究的目的:

为冷解精子适应马类IVF和细胞内精子注射 (ICSI) 协议.
评价精子容量的对胚胎细胞产生的影响和马类胚胎中euploidy率.

主要方法:

卵子接受了用冷解精液进行体外受精,用容量化冷解精液 (ICSI上限) 进行ICSI,或用洗净的冷解精液进行对照ICSI.
使用ANOVA比较了裂变和胚胎囊的发生率.
浅层全基因组测序分析了IVF和ICSI帽子组胚胎中的阳性积分.

主要成果:

使用冷解精液进行试管婴儿的结果是与对照ICSI (16.4%) 相比,更高的胚胎细胞发育率 (22.4%).
在ICSI上限,每次切割胚胎 (69.0%) 和每次卵细胞 (27.8%) 的胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胎胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚胚卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵卵.
试管婴儿和ICSI上限组之间没有观察到胚胎细胞形成日或动脉积分率的显著差异.

结论:

在ICSI或IVF的条件下用冷解的精子化精子可以改善马类胚胎的生产.
这种技术提供了一种新的方法来提高马类体外生产 (IVP) 胚胎的临床效率.
这种方法不会对胚胎无体积率产生不利影响,这表明它对于临床应用的安全性.

关键词:

美国国务院调查局 (ICSI)在这个过程中,IVF IVF.一个宏体的宏体.马类的马类动物.浅层的全基因组测序.

更多相关视频

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Zygotic Fluorescence Recovery After Photo-bleaching Analysis for Chromatin Looseness That Allows Full-term Development

Published on: June 12, 2018

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Published on: July 10, 2021

相关概念视频

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

Meiosis II

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...

Fertilization

Fertilization

During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...