细菌物种的相容性增强了VOC介导的对Botrytis cinerea的系统性耐药性
Abdur Rashid Khan1,2,3, Qurban Ali4, Xuxiang Wu1,2
1The Sanya Institute of Nanjing Agricultural University, Sanya, Hainan Province, 572024, China.
World journal of microbiology & biotechnology
|October 29, 2025
概括
兼容的细菌菌株使用挥发性有机化合物 (VOC) 来触发植物对灰色菌 (Botrytis cinerea) 的防御. 这些挥发性有机化合物有效抑制真菌生长并保护番茄植物,提供了一个有希望的环保生物控制策略.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 生物控制生物控制
背景情况:
- 在全球范围内,Botrytis cinerea在番茄生产中造成了重大损失.
- 来自微生物的挥发性有机化合物 (VOC) 可以诱导植物的耐药性.
- 在VOC介导的植物防御中,Bacillus菌株相容性的作用还没有得到充分的研究.
研究的目的:
- 调查由兼容和不兼容的细菌菌株产生的VOCs对番茄灰菌的疗效.
- 阐明导致VOC引起的系统性耐药性 (ISR) 的机制.
主要方法:
- 针对B. cinerea. 的兼容和不兼容的细菌菌株 (LNXM12,GBAC46,LLTC93) 测试的VOC.
- 利用扫描电子显微镜 (SEM) 来观察真菌的结构变化.
- 评估了反应性氧物种 (ROS),甲 (MDA) 和过氧化 (H2O2) 的水平.
- 进行了与防御相关基因的基因表达分析.
- 进行了脱落的叶子测定和VOC分析.
主要成果:
- 来自兼容和不兼容的Bacillus菌株的VOC抑制了B. cinerea菌株的生长,兼容菌株显示出更强的抑制 (54.05%与42.10%对比).
- SEM显示了B. cinerea hyphae的结构损伤,与相容菌株更为严重.
- 兼容的VOC导致了更高的ROS积累,并调节了所有六个测试的防御基因 (PR1,PR3,MPK6,LOX1,EF1,PAL).
- 兼容和不兼容的VOC在脱落的叶子试验中减少了灰色菌的严重性,并降低了MDA和H2O2水平.
结论:
- 菌株相容性对于VOC介导的抗真菌活性和西红中的ISR诱导至关重要.
- 兼容的Bacillus菌株产生了更广泛的VOC,增强了它们的生物控制潜力.
- 兼容的Bacillus菌株是对灰色菌有希望的环保生物控制剂.
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