从简化的单片片对比不同采样方法的可比舌头涂层微生物群概况:试点研究
Yiwen Li1,2, Mengmeng Zhu2, Wenting Wang2
1Beijing Key Laboratory of Traditional Chinese Medicine Basic Research On Prevention and Treatment for Major Diseases Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Clinical oral investigations
|October 29, 2025
概括
一个单一的舌头擦除用无菌抹布有效地捕获舌头涂层微生物群,类似于多个擦除. 这种简单的方法提供了一种可复制和具有成本效益的方法,用于在健康成年人中进行标准化微生物群采样.
科学领域:
- 微生物组研究的研究.
- 口腔健康 口腔健康
- 系统性疾病的生物标志物
背景情况:
- 舌头涂层微生物群是系统性疾病的潜在生物标志物.
- 缺乏标准化的采样方案阻碍了临床应用和交叉研究比较.
- 试点研究评估不同舌头涂层采样方法的可靠性.
研究的目的:
- 评估不同舌头涂层微生物群采样方法的可靠性.
- 为了确定单个划痕是否会产生与多个划痕可比的结果.
- 确定一个实用的和可重复的采样协议.
主要方法:
- 采集了健康成年人的舌头涂层样本,使用四种方法 (单/多次擦除,擦拭/擦除).
- 进行了元基因组测序来分析微生物多样性,分类组合和功能概况.
- 分析了DNA质量,α/β多样性,属/物种丰富性和KEGG功能预测.
主要成果:
- 单片除方法显示了与多片除方法相似的微生物概况,α-多样性和β-多样性.
- 一致的分类组合 (占主导地位的属:Streptococcus,Prevotella,Rothia) 和功能途径的最小变化.
- 结果表明舌头涂层微生物群的空间稳定性和代表性采样潜力.
结论:
- 使用无菌片进行单次擦除是一种实用,可重复和经济高效的采样方法.
- 这种简化方法在健康成年人中产生了具有代表性的舌头涂层微生物组数据.
- 为了临床应用和标准化,需要在更大,更多样化的队列中进一步验证.
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