ZNFX1作为补偿的dsRNA识别受体,在色斑点中发挥抗病毒作用
Rui Zheng1, Siyou Huang1, Yiling Kang1
1State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), China-ASEAN Belt and Road Joint Laboratory on Mariculture Technology, Guangdong Provincial Key Laboratory of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China.
鱼类中含有1 (ZNFX1) 的指NFX1型作为dSRNA传感器,补偿缺席的RIG-I受体. 它根据细胞类型独特调节抗病毒反应,为鱼类免疫力和病毒对策提供了洞察力.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 水生生物学 水生生物学
背景情况:
- 哺乳动物指NFX1-类型含有1 (ZNFX1) 通过dsRNA识别和RIG-I类受体 (RLR) 路径激活对抗病毒防御至关重要.
- 鱼类ZNFX1对抗病毒免疫的功能在很大程度上是未知的,特别是考虑到许多Actinopterygii (射线鱼) 缺乏RIG-I.
研究的目的:
- 为了识别和表征Epinephelus coioides (EcZNFX1) 中ZNFX1的鱼类正体.
- 调查 EcZNFX1 在抗病毒防御中对色斑点群虫神经亡病毒 (OGNNV) 的作用.
- 阐明 EcZNFX1 在不同鱼类细胞类型中的情境依赖性免疫调节功能.
主要方法:
- 鉴定和表征 EcZNFX1.1.
- 在OGNNV感染时对EcZNFX1表达的分析.
- 试验室试验评估 EcZNFX1 与病毒dRNA 的结合及其与信号分子 (TBK1,IRF3) 的相互作用.
- 评估 EcZNFX1 对不同细胞系 (GB 和 GS 细胞) 的 IFN-I/ISG 生产和 pyroptosis 的影响.
主要成果:
- 鉴定出 EcZNFX1 是一种受干扰素刺激的 dsRNA 传感器,由 OGNNV 感染升高调节.
- EcZNFX1结合病毒dSRNA中间体,并限制病毒在群群细胞中的复制.
- EcZNFX1具有双重的免疫调节作用:抑制神经毒性脑细胞 (GB) 中的抗病毒反应,促进细胞 (GS) 中的IFN-I信号传递.
- EcZNFX1与TBK1相互作用,激活GS细胞中依赖IRF3的IFN-I信号传递.
结论:
- 在RIG-I缺陷的teleosts中,EcZNFX1作为补偿的dsRNA传感器.
- EcZNFX1的抗病毒功能是特定于细胞类型的,证明了依赖上下文的免疫调节.
- 这项研究提供了关于鱼类天生的抗病毒免疫力和水生病毒对策的潜在目标的关键机制见解.
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