集体机械记忆被多细胞球体中的长期超细胞细胞骨架结构编码.
Jiwon Kim1, Hyuntae Jeong1, Young-Woo Jeon1
1Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea.
ACS applied materials & interfaces
|October 29, 2025
概括
集体细胞通过actin-cytoskeleton网络保留他们环境的"机械记忆". 这种细胞记忆会影响细胞行为,影响诸如癌症转移等过程.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 癌症研究 癌症研究
背景情况:
- 动物细胞表现出"机械记忆",根据环境硬性改变形式和功能.
- 以前的研究将这种记忆归因于单个细胞的表观遗传和转录变化.
- 在集体细胞中保留机械记忆的方法在很大程度上是未被探索的.
研究的目的:
- 为了研究集体细胞是否保留机械记忆.
- 阐明集体细胞机械记忆背后的机制.
- 探索集体机械记忆在癌症转移中的作用.
主要方法:
- 模拟卵巢癌转移的模型.
- 在不同硬度的基板上培养细胞 (软原料与硬原料).
- 分析多细胞球形形成和细胞骨结构 (actin-CK18网络).
- 调查差距结点在维护集体记忆中的作用.
主要成果:
- 集体细胞通过自我组织的actin-CK18网络维持机械记忆.
- 软原料细胞表现出更强的细胞-细胞粘附,并在球状体中形成了外围细胞骨.
- 这些子阻碍了集体入侵,限制了球形运动.
- 间隙结的抑制扰乱了细胞骨形成,突出了细胞间通信的作用.
结论:
- 集体细胞拥有独立于仅仅表观遗传/转录因素的机械记忆机制.
- 超细胞actin-CK18网络和细胞间通信对于集体机械记忆至关重要.
- 这一发现提升了对转移性过程中瘤细胞集群行为的理解.
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