在后列注入标记的 Racemic Chiral 选择器,使得 LC-Chiral MS/MS 成为可能
Xingcheng Gong1,2, Yang Yang1,2, Lijuan Wu1,2
1Modern Research Center for Traditional Chinese Medicine, Beijing Research Institute of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Analytical chemistry
|October 29, 2025
概括
一种新方法使用标记的性选择器 (CS) 与后列输液LC-MS/MS,用于同时确定反体过量和性识别. 这种多功能工具通过使复杂样本中精确的反体量化,有助于奇拉代谢.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 代谢学 代谢学 代谢学
背景情况:
- 精确的反体过量 (ee) 确定对于分析复杂矩阵中的性化合物至关重要.
- 现有的性分析方法通常需要单独的步骤来进行性识别,性选择器 (CS) 选和EE确定.
- 为这些任务制定统一的方法可以显著简化性代谢学研究.
研究的目的:
- 开发一种单一的管道,用于使用LC-MS/MS进行合性识别,CS查和EE测定.
- 为了能够在复杂的生物样本中测量反体.
- 为了建立一种多功能工具,用于性代谢学,甚至对于缺乏特定功能组的代谢物.
主要方法:
- 在Cu2+的存在下,利用了标记的赛性选择器 (dl-Phe) 的后列输液 (PCI).
- 从反体生成二聚体三聚体离子,使得基于差异解离动力学的性MS/MS分析成为可能.
- 采用完整的碰撞能量 (FCER) -MS2光谱用于奇拉识别和与热度相关的离子强度.
主要成果:
- 成功实现了从Peucedani Radix (PR) 获得的角类型皮拉诺库马林 (AP) 反体的性识别.
- 根据复杂的形成和独特的解离模式,选了15个AP作为潜在的性选择器.
- 使用开发的方法,在茶样和PR中的AP中同时确定氨基酸 (AAs).
结论:
- 标记的种族性CS和PCI-LC-MS/MS的整合为表态分子测量提供了"三只鸟用一块石头"的解决方案.
- 这种方法为性代谢学提供了一种多功能和高效的工具,适用于广泛的代谢物.
- 这种方法有助于在代谢学研究中识别异构体作为关键差异变量.
更多相关视频
09:36Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
Published on: March 8, 2024
1.4K
11:00Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
23.3K
相关概念视频
02:30Racemic Mixtures and the Resolution of Enantiomers
21.2K
A racemic mixture, or racemate, is an equimolar mixture of enantiomers of a molecule that can be separated using their unique interaction with chiral molecules or media. Racemic mixtures are denoted by the (±)- prefix. This ‘optical rotation descriptor’ applies to the whole solution of a racemic mixture rather than a specific stereoisomer. Enantiomers typically have the same physical and chemical properties. Hence, they are not easily separable. However, enantiomers can exhibit...
21.2K
02:15SN1 Reaction: Stereochemistry
10.1K
This lesson provides an in-depth discussion of the stereochemical outcomes in an SN1 reaction.
In the first step of an SN1 reaction, the bond between the electrophilic carbon and the leaving group ionizes to generate the carbocation intermediate. The second step of the mechanism is the nucleophilic attack.
In the formed carbocation, the positively charged carbon is sp2 hybridized with a trigonal planar geometry. As all the three substituents lie on the same plane, a plane of symmetry for the...
In the first step of an SN1 reaction, the bond between the electrophilic carbon and the leaving group ionizes to generate the carbocation intermediate. The second step of the mechanism is the nucleophilic attack.
In the formed carbocation, the positively charged carbon is sp2 hybridized with a trigonal planar geometry. As all the three substituents lie on the same plane, a plane of symmetry for the...
10.1K
01:05High-Performance Liquid Chromatography: Elution Process
1.4K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
1.4K
02:05Prochirality
4.8K
The concept of prochirality leads to the nomenclature of the individual faces of a molecule and plays a crucial role in the enantioselective reaction. It is a concept where two or more achiral molecules react to produce chiral products. A typical process is the reaction of an achiral ketone to generate a chiral alcohol. Here, the achiral reactant reacts with an achiral reducing agent, sodium borohydride, to generate an equimolar mixture of the chiral enantiomers of the product. For example, an...
4.8K
01:11High-Performance Liquid Chromatography: Introduction
3.3K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
3.3K
00:57High-Performance Liquid Chromatography: Instrumentation
2.9K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.9K