相关实验视频
Updated: Jun 6, 2026

13:04
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
Published on: March 1, 2019
确保:抑制器tRNA的百科全书与人工智能助理
Zhuo Ouyang1, Yifeng Zhang1, Fan Feng1
1MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, Innovation Center for Evolutionary Synthetic Biology, Guangdong Provincial Key Laboratory of Plant Stress Biology, School of Agriculture and Biotechnology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Nucleic acids research
|October 29, 2025
概括
抑制器转移RNAs (sup-tRNAs) 能够读取过早停止的子,以挽救截断的蛋白质. 新的ENSURE平台集成了sup-tRNA数据,结构模型和人工智能助理,以加速研究和治疗开发.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物信息学是一种生物信息学.
背景情况:
- 抑制器转移RNAs (sup-tRNAs) 对于通过转化读透来拯救具有过早终结密码子的蛋白质至关重要.
- 遗传密码扩展和RNA疗法的进步刺激了sup-tRNA工程,但缺乏统一的数据平台.
研究的目的:
- 引入ENSURE,这是一个全面的知识库,旨在整合各种与sup-tRNA相关的数据.
- 通过提供先进分析工具的集中资源,促进sup-tRNA研究和翻译应用.
主要方法:
- 汇总了2152种与疾病相关的变异,86种天然的亚tRNA,1108种tRNA工程策略和487种tRNA元素记录.
- 应用多个序列对齐,二次结构预测和AlphaFold 3建模与交互式可视化.
- 使用检索增强生成来增强数据查询和答案生成的人工智能助理的开发.
主要成果:
- ENSURE提供了一个精心策划的sup-tRNA信息数据库,包括变体,自然的sup-tRNA,工程策略和tRNA元素.
- 集成的结构建模和可视化工具为tRNA结构和功能提供了深入的见解.
- 人工智能助理有效处理查询,检索相关信息,并通过集成资源生成信息化的答案.
结论:
- 恩苏尔 (ENSURE) 作为一个强大的,对sup-tRNA研究的整合性平台.
- 该平台的综合数据,结构建模和人工智能能力预计将加速基于sup-tRNA的治疗方法的开发.
- ENSURE解决了对专门资源的需求,以推进翻译阅读和RNA治疗领域的发展.
相关概念视频
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
tRNA Activation
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
Nonsense-mediated mRNA Decay
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Non-equilibrium in the Cell
An important concept in studying metabolism and energy is that of chemical equilibrium. Most chemical reactions are reversible. They can proceed in both directions, releasing energy into their environment in one direction, and absorbing it from the environment in the other direction. The same is true for the chemical reactions involved in cell metabolism, such as the breaking down and building up of proteins into and from individual amino acids, respectively. Reactants within a closed system...
tRNA Activation
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...

