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相关概念视频

mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

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The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
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Nonsense-mediated mRNA Decay02:27

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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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相关实验视频

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Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
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TTDB:一个全面的转录组周转数据库,用于探索mRNA稳定性.

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  • 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangzhou 510060, China.

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概括

转录组周转数据库 (TTDB) 集中了来自众多研究的mRNA衰变率数据. 这个资源有助于研究人员了解五种物种的基因表达调节.

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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 使者mRNA的周转对于调节基因表达水平至关重要.
  • 高通量测序产生了广泛的转录组范围的mRNA衰变数据.
  • 现有的mRNA稳定性数据在许多出版物中分散,阻碍了分析.

研究的目的:

  • 为全基因组mRNA稳定性数据创建一个集中,全面的资源.
  • 为了促进mRNA衰变速率的协调和比较.
  • 提供一个用户友好的平台来访问和分析mRNA周转信息.

主要方法:

  • 整合了来自57个出版物的198个高通量数据集.
  • 标准化mRNA半衰期和衰变率在计算上.
  • 收集了五种物种的数据:人类,老鼠,斑马鱼,果和酵母.

主要成果:

  • 开发了转录组周转数据库 (TTDB).
  • 每个数据集的TTDB包括平均12544个基因的数据.
  • 为成绩单提供预先计算的注释,包括GC内容和监管元素.

结论:

  • TTDB为mRNA稳定性数据提供了一个有价值的,集中的资源.
  • 该数据库简化了对基因表达调节的研究.
  • TTDB是通过网络界面公开访问的,用于广泛的研究.