由TAMs衍生的IL-1β诱导的DDX21通过JAK2/STAT3通路增强CRC增殖和转移
Yu Wang1, Tiantian Zhen1, Shujin He1
1Department of Pathology, the First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.
Cancer treatment and research communications
|October 29, 2025
概括
瘤相关巨细胞 (TAMs) 通过分泌IL-1β促进结直肠癌 (CRC) 转移,从而增强DDX21表达和瘤进展. 向IL-1β为CRC提供了一个潜在的免疫疗法策略.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 瘤相关巨细胞 (TAMs) 是瘤微环境中的关键参与者,通常与结直肠癌 (CRC) 扩散和转移有关.
- 以前的研究确定了DDX21在CRC转移中的作用,但其与瘤微环境的联系尚不清楚.
研究的目的:
- 研究TAMs在结直肠癌 (CRC) 进展中的生物学作用.
- 阐明连接TAMs,DDX21和CRC转移的分子机制.
主要方法:
- 使用RT-qPCR,迁移/入侵试验,免疫组织化学,生物信息学,西部斑,ELISA,瘤球,殖民地形成,Co-IP和体内实验.
- 分析了TAMs衍生因素对CRC细胞和DDX21表达的影响.
- 研究了JAK2/STAT3通路和DDX21蛋白质的稳定性.
主要成果:
- 在CRC中,受CRC条件的巨细胞增加了DDX21的表达,增殖,迁移,入侵和干性.
- 由TAM衍生的IL-1β激活了JAK2/STAT3,增强了DDX21蛋白的稳定性并增加了ZE1,从而促进了CCL8的产生和巨细胞的招募.
- 抑制CCL8或IL-1β减少了巨细胞迁移和CRC转移;在TAM中IL-1β和CD206的表达与CRC恶性病变和预后相关.
结论:
- 由TAM衍生的IL-1β通过JAK2/STAT3/DDX21和ZEB1通路驱动CRC干,迁移和入侵,诱导CCL8进行巨细胞招募.
- 向TAM分泌的IL-1β是CRC免疫疗法的有希望的候选人.
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