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综合性转录和基因组分析揭示了在甘德斯的精液质量稳定背后的监管机制

Shenqiang Hu¹, Jiasen Chen¹, Xiaoyong He¹

¹State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.

Poultry science

|October 29, 2025

概括

甘精液质量的稳定性对于生殖效率至关重要. 这项研究确定了关键基因 (EP300,RAB31,PRKG1,PIK3R1) 和影响精液质量的途径,为水禽繁殖计划提供了见解.

科学领域:

动物科学动物科学
生殖生物学生殖生物学
遗传学是一个遗传学.

背景情况:

的生殖性能受到精液质量的显著影响.
季节性繁殖者,种植者经常经历精液质量下降,基础机制不太清楚.
保持稳定的精液质量对于水禽生产效率至关重要.

研究的目的:

系统地调查不同产卵阶段的羊精液质量参数.
确定调节精液质量稳定的分子机制.
为水禽的遗传选择和生殖管理提供参考资料.

主要方法:

精液质量评估和早期,高峰期和晚期产卵阶段的体组织形态学分析.
转录和选择性扫描分析以确定关键基因.
蛋白质与蛋白质相互作用网络的构建和RT-qPCR验证.

主要成果:

与不稳定的群体相比,具有稳定的精液质量的雄鹿表现出更高的参数 (例如精子密度) 和更大的丸.
四个枢纽基因 (EP300,RAB31,PRKG1,PIK3R1) 和相关的KEGG通路 (附着结,间隙结等) 已经确定了.
特定的基因表达模式 (例如,不稳定时更高的EP300,RAB31,PRKG1;稳定时更高的PIK3R1) 与精液质量稳定性有关.

结论:

提供了新的洞察力,了解在繁殖季节期间子精液质量稳定性的分子机制.
EP300,RAB31,PRKG1和PIK3R1的基因表达模式与精液质量稳定性有关.
这些发现为水鸟育种中的遗传选择和生殖管理提供了有价值的参考资料.

关键词:

子子子子选择性的扫描分析.测试 (Testis) 是一个测试.精液质量精液质量转录基因分析

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