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血管内皮生长因子含有从暴露于PFOS的人类颗粒细胞的条件介质促进了体外内皮血管生成

Tamara Tomanic¹, Dragana Samardzija Nenadov¹, Bojana Stanic¹

¹University of Novi Sad, Faculty of Sciences, Department of Biology and Ecology, Serbia.

|October 29, 2025

概括

在人体颗粒细胞中暴露于 perfluorooctanesulfonic acid (PFOS) 会增加血管内皮生长因子A (VEGFA) 的分泌. 这种分泌的VEGFA促进血管生成,为PFOS提供了新的见解.

科学领域:

环境毒理学环境毒理学
生殖生物学生殖生物学
细胞信号传输细胞信号传输

背景情况:

perfluorooctanesulfonic acid (PFOS) 是一种持久性环境污染物,与生殖健康问题有关.
人类颗粒细胞 (HGrC1) 对于卵巢功能和卵泡发育至关重要.

研究的目的:

为了研究PFOS对HGrC1细胞的急性影响.
阐明血管内皮生长因子A (VEGFA) 在PFOS介导的细胞反应中的作用.

主要方法:

暴露HGrC1细胞在不同度的PFOS中.
全球mRNA测序和蛋白质分析.
研究包括PI3K/AKT和PKA在内的信号通路.
评估内皮细胞迁移和管道形成.

主要成果:

在HGrC1细胞中的PFOS上调 VEGFA mRNA,最大诱导在25μM.
暴露于PFOS激活了AKT和CREB信号通路.
PFOS降低了细胞内VEGFA,但增加了它的分泌.
来自暴露于PFOS的细胞的条件介质促进了由VEGFA介导的内皮细胞迁移和管形成.

结论:

通过VEGFA,PFOS在内皮细胞中诱导了亲血管效应.
维格法是PFOS对卵巢功能作用的关键媒介.
这些发现为PFOS毒性机制提供了新的见解.

关键词:

血管新生的产生.人类颗粒体细胞 HGrC1 这就是为什么PFOS是PFOS.在VEGF中,VEGF是VEGF.

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