内皮细胞衍生的细胞外囊在调节纤维细胞功能中的作用 在皮肤伤口愈合中的作用
Heidi Yuan1, Anna M Salapatas2, Trevor R Leonardo2
1Department of Integrative and Translational Physiology, College of Medicine, University of Illinois Chicago, Chicago, Illinois, USA; Center for Wound Healing and Tissue Regeneration, University of Illinois Chicago, Chicago, Illinois, USA.
The Journal of investigative dermatology
|October 29, 2025
概括
内皮细胞衍生的细胞外囊泡 (ECEVs) 与纤维细胞通信,增强伤口愈合. 这些ECEVs促进纤维细胞增殖和细胞外基质的产生,这对于组织修复至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 组织修复机制 组织修复机制
- 细胞外囊泡生物学 细胞外囊泡生物学
背景情况:
- 伤口愈合涉及内皮细胞和纤维细胞之间的复杂细胞间通信.
- 在颗粒组织形成过程中,内皮细胞和纤维细胞共同定位.
- 内皮细胞衍生细胞外囊泡 (ECEVs) 在调节纤维细胞行为的作用仍然在很大程度上未被探索.
研究的目的:
- 在体外和体内研究ECEVs对纤维细胞行为的功能影响.
- 阐明ECEVs在伤口修复过程中影响纤维细胞活动的分子机制.
主要方法:
- 用ECEVs治疗的纤维细胞的转录组分析.
- 在体外功能测试评估纤维细胞增殖,细胞循环和细胞外基质沉积.
- 在体内小鼠皮肤伤口愈合模型与ECEV的管理.
主要成果:
- ECEV治疗改变了与细胞分裂,细胞外矩阵组织和纤维化有关的纤维细胞基因表达.
- 调节ETV1转录因子介导的纤维细胞对EGCV中存在的FGF2的反应.
- 在体外,ECEVs增强了纤维细胞增殖,细胞循环进展,并改变了ECM沉积.
- 在体内,ECEV的使用增加了伤床中的原蛋白密度和纤维细胞数量.
结论:
- ECEVs代表了一种新的传播途径,它调解了内皮细胞对纤维细胞功能的影响.
- 在促进有效的伤口修复所必需的关键细胞过程中,ECEVs起着重要作用.
- 这项研究确定了在组织再生过程中调节纤维细胞活动的以前未知的机制.
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