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生物传感器的进步用于氨基酸及其衍生物的微生物生物合成

Huina Dong¹, Tingting Li², Xinyi Ren³

¹State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China; University of Chinese Academy of Sciences, Beijing, China.

Biotechnology advances

|October 29, 2025

概括

本综述详细介绍了用于选氨基酸生产微生物菌株的直接和间接生物传感器. 这些工具对于推动生物技术和可持续代谢物生产至关重要.

科学领域:

代谢工程是代谢工程.
合成生物学合成生物学
生物技术是生物技术.

背景情况:

氨基酸和衍生品在生物技术,制药,农业和制造业中至关重要.
高通量选方法对于识别关键酶和过度生产氨基酸生产菌株至关重要.

研究的目的:

系统地审查和分析直接和间接的生物传感策略,用于氨基酸生产菌株的代谢工程.
批判性地评估各种生物传感器类的设计,优化和实施.
为了比较不同的生物传感方法,并确定代谢工程应用的最佳参数.

主要方法:

对六类直接生物传感器 (基于转录因子, рибо开关,FRET,cpFP,可诱导促进剂,翻译元件) 的系统评估.
对四类间接生物感应策略 (准代谢前体,下游代谢产物或中间体) 的系统分析.
运营原则的批判性分析,合理设计,性能优化和实际实施.

主要成果:

审查的直接生物传感器包括转录因子,核糖开关,FRET,cpFP,可诱导的促进物和翻译元素.
间接生物感应策略侧重于通用/特定路径的前体,下游代谢物或分支路径中间体.
对现有生物传感器的设计策略,优化方法和实施挑战进行比较.

结论:

这一审查有助于开发新的生物传感器,用于缺乏当前系统的代谢物.
它促进现有生物传感器技术的创新,以提高效率和可持续性.
这些发现支持有效和可持续地生产氨基酸相关化合物和其他高价值代谢物.

关键词:

氨基酸是氨基酸中的一种.氨基酸衍生物氨基酸衍生物直接使用生物传感器.高通量选的高通量选间接生物传感器是一种间接生物传感器.代谢工程是代谢工程.

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