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使用ISSR标记物对咖叶基因型的分子表征

Dharini Chittaragi¹, M Ananthan², K Venkatesan³

¹Department of Spices and Plantation Crops, Tamil Nadu Agricultural University, Coimbatore, 641003, India. chitti.smile3@gmail.com.

Scientific reports

|October 30, 2025

概括

咖叶 (Murraya koenigii) 的遗传多样性使用简单序列重复 (ISSR) 标记物进行了评估. 这项研究揭示了显著的变异性,这对于改善南印度咖叶种植和生殖质管理至关重要.

科学领域:

* 农业科学农业科学
* 植物遗传学
* 分子生物学 * 分子生物学

背景情况:

*Murraya koenigii (咖叶) 是来自鲁塔科植物家族的重要树香料.
* 变性评估对于作物改进计划至关重要.
*南印度咖叶基因型需要对遗传多样性的评估.

研究的目的:

* 评估南印度咖叶基因型之间的遗传变异性.
* 用简单序列重复 (ISSR) 标记器对咖叶加入进行分析.
*为咖叶的生殖血管理和育种策略提供信息.

主要方法:

*来自南印度各个地方的30个咖叶接入的集合.
*使用13个简单间序列重复 (ISSR) 标记物进行分子分析.
*使用多态信息内容 (PIC) 值和树状图集群的基因多样性分析.

主要成果:

*高平均PIC值 (0.83) 表示通过ISSR标记器有效地揭示多样性.
*UBC-856,UBC-807,UBC-834和UBC-835标记物显示PIC值>0.5.5,其中PIC值>0.5
*基因型根据相似性指数被分为九个群,其中I群是最大的 (15个基因型).

结论:

*在使用ISSR标记物的咖叶基因型中观察到显著的遗传变异性.
*研究结果支持有效的生殖质管理和优质基因型的选择用于繁殖.
*这项研究为南印度的咖叶保护和种植策略提供了基础.

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