血激活介质通过MAPKs途径诱导口腔状细胞癌的亡
Hun Jang1, Yoon-Seo Jang1, Gyoo-Cheon Kim2,3
1Department of Oral and Maxillofacial Surgery, Dental and Life Science Institute, Dental school, Pusan National University, Yangsan, 50612, Republic of Korea.
Scientific reports
|October 30, 2025
概括
血激活介质 (PAM) 在体内有效降低了口腔癌细胞活力和瘤生长. 这种癌症治疗替代方案通过线粒体途径诱导亡,比直接的血应用提供了潜在的优势.
科学领域:
- 生物医学工程 生物医学工程
- 在瘤学瘤学.
- 等离子体物理学的物理学
背景情况:
- 没有臭氧的冷血 (NCP) 正在探索用于癌症治疗,而激活血介质 (PAM) 提供了比直接方法更好的组织透和方便.
- 口腔状细胞癌 (OSCC) 在治疗中存在挑战,需要新的治疗策略.
研究的目的:
- 评估血激活介质 (PAM) 在体外和体内对口腔状细胞癌 (OSCC) 的疗效和潜在机制.
- 评估PAM对癌细胞的选择性与正常角质细胞相比.
主要方法:
- 在体外研究中使用了SCC-25 (OSCC) 和HaCaT (角质细胞) 细胞,评估活力 (SRB),细胞骨变化 (F-actin),细胞亡标记物 (细胞染色体C,AIF,西斑分裂酶3,PARP,Bax,bcl-2) 和MAPK信号 (JNK,p38,ERK).
- 在体内研究涉及SCC-25异种移植裸体小鼠模型,接受PAM治疗,监测瘤体积,体重,并进行组织学分析 (H&E,TUNEL).
主要成果:
- 通过促进细胞染色体C和AIF的线粒体转移,PAM显著降低了SCC-25细胞活力,破坏了F-actin细胞骨架,并诱导了细胞亡.
- 西方斑点通过内在途径 (切割PARP/caspase3,改变Bax/bcl-2比率) 和调制MAPK信号 (增加JNK/p38酸化,减少ERK酸化) 证实了PAM诱导的亡.
- 在体内,PAM治疗使瘤体积减少32%,瘤重量减少25%,而不影响小鼠体重,瘤细胞亡显著增加 (TUNEL试验).
结论:
- 血激活介质对口腔状细胞癌细胞具有选择性细胞毒性,通过线粒体内在途径和MAPK信号传递诱导亡.
- PAM为OSCC提供了直接血治疗的有希望的替代方案,克服了组织透和处理等局限性.
- 建议进行更多涉及途径抑制的研究,以充分阐明PAM诱导的亡机制.
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