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使用生物信息学方法在儿科克罗恩病中识别与铁死相关的基因

Peng Lin¹, Shuxia Xu², Xiaoxiao Yan¹

¹Department of Pathology, Fujian Children's Hospital (Fujian Branch of Shanghai Children's Medical Center), College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, 350000, China.

Scientific reports

|October 30, 2025

概括

铁死在儿科克罗恩病 (PCD) 中起着关键作用. 这项研究确定了关键的铁灭基因和免疫细胞,揭示了IL1B作为PCD的有希望的诊断标志物.

科学领域:

生物医学研究的研究.
基因组学就是基因组学.
免疫学免疫学免疫学

背景情况:

铁亡是炎症性肠病 (IBD) 的关键机制.
了解儿科克罗恩病 (PCD) 中的铁死对于向治疗至关重要.

研究的目的:

在PCD中确定关键的铁亡相关基因 (FRG).
分析PCD中失调的信号通路和免疫透.
评估已识别的基因的诊断潜力.

主要方法:

对基因表达数据的综合生物信息学分析 (GSE117993).
来自FerrDb.的与铁死相关的基因 (FRG) 治愈.
蛋白与蛋白相互作用 (PPI) 网络分析.
免疫细胞透评估 (ssGSEA). 免疫细胞透评估.
接收器操作特征 (ROC) 曲线分析用于验证.

主要成果:

确定了1074个差异表达基因 (DEG),包括21个FRG-DEG.
丰富分析将FRG-DEG与代谢调节和IL-17信号联系起来.
已经发现了五个枢纽基因:PTGS2,IFNG,IL1B,IDO1,NOS2.
观察到骨髓衍生抑制细胞 (MDSC) 和中性粒细胞的显著积累.
在验证队列中,IL1B显示了最高的诊断性能.

结论:

这项研究为PCD中的铁亡机制提供了新的见解.
已识别的枢纽基因,特别是IL1B,可以作为潜在的诊断生物标志物.
这些发现支持推进儿科克罗恩病的精密疗法.

关键词:

生物信息学是一种生物信息学.诊断生物标志物诊断生物标志物铁化是铁化的一种.儿童克罗恩氏病儿童克罗恩氏病

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