相关实验视频

Updated: Jan 13, 2026

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens

Author Spotlight: Optimized Transformation Protocol for Chlorella vulgaris Using Agrobacterium tumefaciens

Published on: October 27, 2023

设计了CRISPR-Cas9载体,用于有效地通过农细菌介导的Phanerochaete chrysosporium的转化

Simran Jatwani¹, Falguni Aggarwal¹, Rishabh Rajkumar Jadhav¹

¹Department of Biotechnology, School of Engineering and Applied Sciences, Bennett University, Tech Zone II, Plot Nos. 8-11, Greater Noida, U.P., India.

Molecular biotechnology

|October 30, 2025

概括

我们优化了Phanerochaete chrysosporium的Agrobacterium介导的转化,这是一种具有高素降解能力的真菌. 这一突破使得基因工程能够用于可持续的生物产品.

科学领域:

菌类学菌类学是指菌类学.
生物技术是生物技术.
合成生物学合成生物学

背景情况:

帕纳罗切特是白色腐烂的真菌,具有显著的解能力.
由于转化效率低,对P. chrysosporium的基因操纵受到阻碍.

研究的目的:

为Phanerochaete chrysosporium开发一个优化的Agrobacterium介导转化 (AMT) 协议.
建立功能性基因组编辑和P. chrysosporium中的代谢工程的基础.

主要方法:

修改一个具有sGFP记者基因的CRISPR-Cas9兼容二元向量.
使用菌根盘和三明治选择方法进行转化.
优化共同种植参数,包括温度和添加乙酸.

主要成果:

实现了携带抗胺素和sGFP基因的T-DNA的稳定整合.
确定了低温化 (23°C) 和乙酸作为提高转化效率的关键因素.
在转变的P. chrysosporium mycelia中确认了sGFP的稳定表达.

结论:

开发的AMT协议显著提高了P. chrysosporium的转化效率.
这项研究提供了基于Cas9的载体和异质基因表达的稳定维护的概念证明.
这种优化的协议对于推进P. chrysosporium的代谢工程至关重要,用于素价值化和可持续生物化学生产的应用.

关键词:

农业细菌介导的转化 (AMT)克里斯普尔-卡斯9基因修饰是一种基因修饰.在真菌转化转化过程中.选择性的三明治涂层技术 sGFP 的基因表达.

更多相关视频

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

CcCIPK14 Gene Function Analysis to Illuminate the Efficient Root Transgenic System

Published on: September 23, 2021

相关概念视频

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...