PEG诱导的液体-液体相分离对DNA-托波特干相互作用的影响
Dineshbabu Takkella1, Jyoti Vishwakarma1, Krishna Gavvala1
1Department of Chemistry, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, Telangana 502284, India.
The journal of physical chemistry. B
|October 30, 2025
概括
聚乙烯甘醇 (PEG) 诱导的液体液相分离 (LLPS) 改变了药物的光物理和DNA结构. 这种分子拥挤通过调整激发状态动态和核酸组织来影响药物输送和生物分子工程.
科学领域:
- 生物物理学的生物物理.
- 材料科学 材料科学 材料科学
- 药物运输 药物运输 药物运输
背景情况:
- 液-液相分离 (LLPS) 对细胞组织和功能至关重要.
- 了解LLPS对小分子和生物宏分子的影响对于先进的应用至关重要.
- 聚乙烯甘醇 (PEG) 是生物分子系统中LLPS的常见诱导剂.
研究的目的:
- 调查PEG诱导的LLPS如何影响托波特干 (TPT) 的光物理性质.
- 检查PEG诱导的LLPS对小牛胸腺DNA (ctDNA) 的结构组织的影响.
- 在二进制 (PEG:TPT,PEG:ctDNA) 和三进制 (PEG:TPT:ctDNA) 系统中探索这些效应.
主要方法:
- 光谱技术 (光,吸收) 用于监测光物理变化.
- 显微镜分析凝结物形态.
- 分子动力学 (MD) 模拟以阐明分子相互作用和结构变化.
主要成果:
- 由PEG诱导的LLPS将TPT从Z形式转移到C-TPT形式,增强光和寿命,并抑制激发状态质子转移 (ESPT).
- LLPS显著改变了ctDNA结构,杀了B型信号,并导致光谱蓝色转移.
- 三级系统表现出类似的TPT行为,尽管DNA结合部分减轻了PEG的影响;MD模拟证实了拥挤并增强了TPT-DNA相互作用.
结论:
- 通过PEG介导的LLPS可以有效调整像TPT这样的小分子的兴奋状态动态.
- LLPS影响核酸如ctDNA的结构组织.
- 研究结果为控制在拥挤环境中的分子行为提供了洞察力,用于药物输送和生物分子工程.
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