牙纸干细胞中的EphrinB2促进内皮细胞在3D生物打印水凝结构中形成毛细血管状绳索
Wen Wang1,2, Junjun Li1, Yingying Zhou1
1School of Stomatology, Xuzhou Medical University, No. 209 Tongshan Road, Xuzhou, Jiangsu, 221004, China.
BMC molecular and cell biology
|October 30, 2025
概括
在3D生物打印水凝中,牙纤维干细胞中的EphrinB2过度表达显著增强了血管形成,3D生物打印水凝,改善了体外带组装和体内血管化.
科学领域:
- 生物材料科学 生物材料科学
- 干细胞生物学 干细胞生物学
- 血管生物学 血管生物学
背景情况:
- 3D生物打印可以创建复杂的组织结构.
- 血管化对于工程组织的生存和功能至关重要.
- 埃弗林B2在3D血管网络形成中的作用需要进一步阐明.
研究的目的:
- 研究ephrinB2在生物打印水凝中的毛细血管状绳索自我组装中的作用.
- 优化牙髓干细胞 (DPSCs) 与人静脉内皮细胞 (HUVECs) 的比例以及凝甲基酸盐 (GelMA) 的功能化程度 (DoF) 以增强带形成.
- 为了评估调节DPSC中的ephrinB2表达对体外和体内血管化的影响.
主要方法:
- 使用共同培养的DPSC和HUVEC通过生物打印制造先前血管化的水凝结构.
- 毛细血管状绳索形态的定量分析,包括分支点和绳索长度.
- 在DPSC中操纵ephrinB2水平 (抑制,淘汰或过度表达) 并评估其对带生成的影响.
- 在小鼠体内进行生物打印水凝的皮下移植以评估血管化.
主要成果:
- 生物打印的水凝成功地形成了带有光线的毛细血管状绳索,由表达细胞周围细胞标记物的DPSC包围.
- 1:3和30%的最佳DPSC:HUVEC比率DoF GelMA显著增加了分支点和电缆长度.
- 抑制或淘汰以弗林B2 损害了绳索形成,而以弗林B2 的过度表达促进了它.
- 在体内研究表明,在DPSC中,用升调的林B2在水凝中的血管数量增加了超过3倍.
结论:
- 在3D生物打印水凝中,EphrinB2在促进毛细血管状绳索形成方面发挥着至关重要的作用.
- 在DPSC中过度表达ephrinB2,增强了体外血管网络的自我组装和体内血管化的作用.
- 这项研究为使用生物打印和干细胞技术设计血管化结构提供了基础.
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