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陪伴者GrpE调解了Mycoplasma bovis中的粘附和与宿主细胞外基质组件和等离子体生成物的相互作用
Xiangrui Jin1,2,3, Huafang Hao1,2,3, Ahmed Adel Baz1,2,3,4
1State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
菌体使用GrpE蛋白质附着于宿主细胞和细胞外基质. 准GrpE可能会导致牛感染的新疗法.
科学领域:
- 兽医微生物学 兽医微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 牛菌会导致牛的重大疾病,如肺炎和乳腺炎.
- 目前的治疗方法受到抗生素耐药性和缺乏疫苗的限制.
- 在M. bovis的病原性中,伴侣GrpE的作用是未知的.
研究的目的:
- 调查GrpE在M. bovis粘附和致病性中的作用.
- 为了确定GrpE是否是治疗干预的潜在目标.
主要方法:
- 在M. bovis菌株中研究了GrpE的存在和定位.
- 评估了M. bovis对具有或没有抗GrpE血清的胚胎牛肺细胞的附着性.
- 分析了GrpE与宿主细胞外基质组件的结合和等离子体激活.
- 产生了一个grpE基因淘汰突变,并为功能分析补充了菌株.
主要成果:
- GrpE存在于M. bovis表面膜上,对于粘附于EBL细胞至关重要.
- 抗GrpE血清抑制了M. bovis的粘附.
- GrpE与宿主细胞外基质蛋白 (纤维蛋白,IV原蛋白,拉米宁,维特龙蛋白) 结合并激活等离子体.
- 破坏grpE显著减少粘附,在补充后恢复了粘附.
结论:
- GrpE是一种新型的M. bovis粘附蛋白,对于宿主ECM相互作用和等离子素激活至关重要.
- 在M. bovis的致病性方面,GrpE起着至关重要的作用.
- 针对GrpE提供了针对M. bovis感染的新型疫苗和治疗策略的潜力.
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