考古体表达IS200/IS605相关 ωRNAs的圆形异型
Beatriz A Picinato1, Vinícius H Franceschini-Santos1, Lívia S Zaramela1
1Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
Frontiers in microbiology
|October 30, 2025
概括
循环RNAs (circRNAs) 在古生物中很丰富,有新的循环 ωRNAs 与移动元素联系在一起. 这项研究开发了一个计算管道,MonArch,以识别这些circRNAs在考古RNA-Seq数据中.
科学领域:
- * 分子生物学 * 分子生物学
- * 基因组学 是一个学科.
- * 生物信息学是一门学科.
背景情况:
- *循环RNAs (circRNAs) 是带有结尾的RNA分子,已知在真核生物中起着调节作用.
- * 转基因RNA在原核生物中研究不足,在古生物中识别有限.
- *以前的研究主要使用真核生物特异的方法进行circRNA识别.
研究的目的:
- *为circRNA识别开发一种 prokaryotic 特定的计算管道 (MonArch).
- * 为了识别和表征考古团中的circRNAs *Halobacterium salinarum*.
- * 调查各种考古物种中circRNAs的流行率和特征.
主要方法:
- *开发MonArch计算管道用于从RNA-Seq数据中检测 prokaryotic circRNA.
- *应用MonArch对*Halobacterium salinarum*circRNA-Seq数据和对20多个公共考古RNA-Seq数据集的重新分析.
- *使用RT-PCR验证已识别的circRNAs.
主要成果:
- * 在H. salinarum*中鉴定了49个高可信度circRNAs,包括与 ωRNAs和IS200/IS605转位酶相关的新型circRNAs.
- *发现了循环 ωRNA 与总 ωRNA 不同的生长依赖的表达模式.
- *在主要考古群体中检测出与rRNA和tRNA处理相关的广泛的circRNAs,表明保存的机制.
- * 鉴定了其他haloarchaeal物种中的圆形 ωRNA,这表明它在IS200/IS605系统中起着保留的作用.
结论:
- *circRNAs在古生物中保存和丰富,超过了之前的估计.
- * MonArch 管道有效地识别出 prokaryotic 的 circRNA.
- * 圆形 ωRNAs 代表了一种新型的古老 circRNAs 类,在移动元素系统和条件表达中具有潜在的作用.
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