从有机废物中分离,生产和优化凝酶
Snehal A Masurkar1, Girish R Pathade1, Shivani B Gujar1
1Department of Biotechnology, Krishna Institute of Allied Sciences, Krishna Vishwa Vidyapeeth "Deemed to be University," Satara, Maharashtra, India.
Journal of pharmacy & bioallied sciences
|October 30, 2025
概括
研究人员从有机废物中分离了凝酶,确定了*Serratia marcescens* OWS4作为一个关键的生产者. 这种细菌分离物在最佳pH 6.6和20°C时表现出显著的凝酶活性.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 酶学 是一种酶学.
背景情况:
- 凝酶在各种生物过程中起着至关重要的作用.
- 从废物来源中分离新的酶提供了可持续的生物技术解决方案.
研究的目的:
- 从有机废物中分离和描述凝酶.
- 为了识别产生显著的凝酶活性的细菌菌株.
主要方法:
- 从有机废弃物中选细菌分离物,使用凝亚格板.
- 通过Ninhydrin方法确认了凝酶活性.
- 分离物体的形态和生化表征.
主要成果:
- 七个细菌分离体显示了凝酶活性.
- 隔离OWS4,被确定为*Serratia marcescens*,表现出最高的凝酶活性 (1.9毫米清晰区域).
- 凝酶活性的最佳条件是pH 6.6和20°C,在24-48小时内产生0.77单位/50毫升.
结论:
- 细菌分离物OWS4 (*Serratia marcescens*) 是一种有前途的凝酶来源.
- 最佳活动条件的表征为酶应用提供了基础.
- 利用有机废物进行酶分离是一种环保的方法.
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