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识别两足动物中的新型DNA吸附体,并使用分散固相提取方法开发用于吸附体的样品准备

Zareen Khan¹, Elena Gorokhova¹, Giulia Martella¹

¹Department of Environmental Science, Stockholm University, Stockholm SE-106 91, Sweden.

Environmental science & technology

|October 30, 2025

概括

研究人员开发了使用两足动物进行环境暴露评估的新DNA adductomics 方法. 分散固相提取 (d-SPE) 改善了样品清洁和检测敏感性,在具有挑战性的矩阵中检测DNA附加物.

科学领域:

环境科学环境科学
分析化学分析化学
毒理学毒理学毒理学

背景情况:

环境DNA附带学对于暴露评估至关重要,但在不同的样本类型中面临着挑战.
使用LC-HRMS进行DNA附带组分析的现有方法在样本清理方面存在困难,特别是在非哺乳动物物种中.
两足动物等物种的状和富含脂质的组织为DNA提取和分析提供了一个困难的矩阵.

研究的目的:

通过集成软件和数据库来增强未知的DNA附带的结构识别.
评估分散固体相提取 (d-SPE) 作为一种有效的清洁方法,用于在甲动物样本中进行DNA adductome分析.
改进DNA吸附学方法,以便在各种环境矩阵中准确评估暴露.

主要方法:

集成的开源软件nLossFinder与DNA补充数据库用于结构性识别补充.
利用精确的质量数据和MS2碎片化来区分核添加物.
引入并评估使用Z-sep+吸附剂进行散射固体相提取 (d-SPE) 进行样本清理.

主要成果:

成功识别了16个DNA添加物,包括两动物DNA的10个新奇修饰.
通过使用d-SPE显著减少矩阵干扰 (例如,脂).
达到了高达170%的LC-HRMS信号响应对DNA adducts的增强,提高了灵敏度.

结论:

综合数据分析工作流和d-SPE为环境监测中的DNA adductomics提供了一个强大的方法.
d-SPE提供了一种简单的,高通量替代传统清洁方法,适合具有挑战性的矩阵.
这些进步使得在各种物种和环境条件下进行更准确,更全面的暴露评估成为可能.

关键词:

在DNA中,添加物是DNA的添加物.分散式固体相提取方法风险曝光评估风险曝光评估高分辨率质谱测量高分辨率质谱非目标分析分析.

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