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Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
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编程细胞凝聚物用于生物材料,使用本质上是无序的蛋白质显示平台.

Rong Chang1, Hann X Tu1, Hongyu Ma2

  • 1Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115.

Proceedings of the National Academy of Sciences of the United States of America
|October 30, 2025
PubMed
概括

我们开发了一个新的平台,用于在细菌上显示内在无序的蛋白质 (IDP),从而实现可编程的自我组织和创建具有可调节性质的动态生物材料.

关键词:
细胞表面显示显示器本质上是无序的蛋白质.生活材料生活材料多细胞模式的形成

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科学领域:

  • 合成生物学 合成生物学
  • 生物材料工程 生物材料工程
  • 蛋白质工程是指蛋白质工程.

背景情况:

  • 自组织对于复杂的生物结构至关重要,但在合成系统中具有挑战性.
  • 内在无序的蛋白质 (IDP) 缺乏稳定的结构,是细胞自我组织的关键.
  • 工程可控制的多细胞材料需要精确控制细胞与细胞的相互作用.

研究的目的:

  • 提供一个可通用的平台,用于对大肠杆菌的高密度IDPs的显示.
  • 使用IDP设计可编程,自我组织的生物材料.
  • 为了证明对细胞凝聚物形成和宏观材料特性的动态控制.

主要方法:

  • 开发了使用CsgF作为E. coli表面显示的支架的内在无序蛋白质显示平台 (iDP^2).
  • 在细菌表面上缺乏稳定的三级结构的融合和呈现的IDP域.
  • 编程具有直角IDP的细胞以实现序列特异性分离并形成动态细胞凝聚物.
  • 研究的环境暗示 (离子强度,温度) 对凝聚液聚合状态的影响.
  • 通过挤出处理的冷凝物产生宏观细丝.

主要成果:

  • 在大肠杆菌上成功显示高密度的IDP,有利于无序序列.
  • 证明具有相分离倾向的IDP驱动动动态细胞凝结物形成.
  • 使用直角的IDP实现了混合细胞群体的序列特异分离,创造了空间有组织的生物组件.
  • 通过环境线索展示了凝结物聚合状态的动态调整性,可从IDP相位行为中预测.
  • 从冷凝物中生成宏观纤维,保持结构完整性和人口隔离.

结论:

  • 该iDP^2平台提供了一个可编程的框架,用于控制细胞与细胞的相互作用和工程生物材料.
  • 这种方法可以设计具有可定制性质的适应性,自我组织的生物系统.
  • 无序的蛋白质图案是创建响应和多细胞工程生物系统的多功能工具.