在多个生命阶段,通过斑马鱼行为终点评估低度圆柱类素的有害影响
Minghui Fu1, Yining Zhang2, Linjie Luo1
1State Key Laboratory for Quality and Safety of Agro - Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Journal of hazardous materials
|October 30, 2025
概括
蓝菌毒素Cylindrospermopsin (CYN) 损害了斑马鱼在生命各个阶段的行为和繁殖. 即使是较低的环境度也会扰乱运动,心率和生殖成功,影响基因表达.
科学领域:
- 环境毒理学环境毒理学
- 生态毒理学 生态毒理学
- 蓝菌毒素研究研究
背景情况:
- 锡林多素 (CYN) 是一种具有全球分布的强有力的蓝菌毒素.
- CYN表现出生物积累和多器官毒性,引发环境和健康问题.
- 了解CYN对水生生物的影响对于风险评估至关重要.
研究的目的:
- 研究环境相关的CYN度对斑马鱼在不同生命阶段的行为和生理影响.
- 要将观察到的表型变化与潜在的分子变化相关联.
- 从行为毒理学的角度提供对CYN毒性的全面了解.
主要方法:
- 斑马鱼胚胎,幼虫和成人的CYN度暴露在0.5至2000μg/L之间.
- 行为评估包括自发运动,游泳表现和求爱行为.
- 生理学测量,如心率,身体长度,产卵,受精率和性激素水平等.
- 斑马鱼幼虫的转录组分析以确定受影响的基因通路.
主要成果:
- 暴露于CYN会减少胚胎的运动,幼虫的游泳能力和成人的求爱行为.
- 生理影响包括胚胎心率减慢,幼虫体长减少,生殖成功减少和成人性激素水平改变.
- 转录组分析显示,与心脏发育,肌肉结构和能量代谢相关的基因发生了显著的变化.
- 在抑制求爱行为和生殖障碍之间发现了强烈的相关性.
结论:
- CYN对斑马鱼的行为和生理学产生显著的负面影响,即使在环境相关的度.
- 观察到的行为缺陷与关键生理系统中潜在的分子变化有关.
- 这项研究强调了CYN的广泛毒理影响,强调了水生生态系统监测和风险管理的必要性.
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