相关实验视频

Updated: Jul 28, 2026

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
12:07

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

16.7K

在微克尺度上,SICyLIA-cTMT以高精度和深度剖析了氧化还原蛋白质组的动态

Sergio Lilla1, Samuel Atkinson2, Sonja Radau3

  • 1Cancer Research UK Scotland Institute, Glasgow G61 1BD, UK.

Cell reports methods
|October 30, 2025
PubMed
概括

科学家们开发了一种新的方法来研究囊氧化,一个关键的细胞信号传递过程. 这种工作流改善了复杂生物样本中这些重要的修改的检测和量化.

关键词:
CP:生物技术 生物技术癌症 癌症 癌症 癌症 癌症囊氨酸的氧化过程纤维细胞的细胞.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量肥胖 肥胖 肥胖 肥胖 肥胖 肥胖 肥胖 肥胖氧化信号传递是一种氧化信号传递.后翻译修改后的修改氧化还原蛋白质组学氧化还原应激的压力.

更多相关视频

Cellular Redox Profiling Using High-content Microscopy11:37

Cellular Redox Profiling Using High-content Microscopy

Published on: May 14, 2017

11.5K
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

2.1K

相关概念视频

Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...