相关实验视频

Updated: Jul 5, 2026

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
14:44

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Published on: September 24, 2012

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序列变性和蛋白沉试验 (SDPP) 提高了在蛋白质层面对连接体标识的灵敏度

Yanni Ma1, Xiaolei Zhang1, Longrun Shi1

  • 1State Key Laboratory of Medical Proteomics, CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, China; University of Chinese Academy of Sciences, Beijing, China.

Analytica chimica acta
|October 30, 2025
PubMed
概括

我们开发了一种新的序列变性和蛋白沉试验 (SDPP),以改善连接体标识. 这种方法通过顺序变质样本来提高灵敏度,放大溶解度转移以获得更好的结果.

关键词:
药物查对象查 药物查对象查体-蛋白相互作用蛋白质组学是指蛋白质组学.顺序变质化是指顺序的变质化.

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相关概念视频

SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...