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扩大时间解析的ICP-MS功能,用于在高盐度介质中表征细胞和生物原纳米颗粒

Nuria Guijarro-Ramírez¹, Raquel González-de Vega², Iraide Sáez-Zamacona³

¹Department of Analytical Chemistry, Nutrition, and Food Sciences, University of Alicante, PO Box 99, 03080 Alicante, Spain.

Analytica chimica acta

|October 30, 2025

概括

由于盐含量高,分析有机生物很困难. 这种新方法使用时间解析感应合等离子体质谱法 (TRA ICP-MS) 来分析单细胞,揭示了Haloferax mediterranei中的生物积累和纳米粒子形成.

科学领域:

分析化学分析化学
环境科学环境科学
微生物学微生物学

背景情况:

通过时间解析的感应合等离子体质谱法 (TRA ICP-MS) 分析类生物是具有挑战性的,因为高总溶解固体 (200 g L-1) 的严重矩阵效应.
在研究中,Haloferax mediterranei被用作模型生物来开发一种新方法来分析高度介质中的性细胞.
现有的方法在这种极端环境中难以保持细胞完整性,并尽量减少矩阵效应.

研究的目的:

引入一种新的TRA ICP-MS方法,用于分析高盐度介质中的单个性细胞.
为了研究 (Pb) 生物积累和纳米粒子 (SeNP) 在单个Haloferax mediterranei细胞中的形成.
为了克服矩阵效应的局限性,并在分析过程中保持细胞完整性.

主要方法:

使用了优化的TRA ICP-MS条件,包括高效的雾化器和双通喷雾室.
在线样品稀释 (1:103比) 和气溶稀释被实施以减少矩阵负载和透应激.
时间解析感应合等离子体飞行时间质谱法 (TRA ICP-TOFMS) 用于检测和量化.

主要成果:

开发的方法成功地保持了细胞完整性,并将高盐基媒体中的矩阵效应降到最低.
对Pb和SeNPs的检测极限分别为15 ag cell-1和42 nm.
单细胞Pb吸收有显著的变化 (20300 ag cell-1),SeNP大小在4773nm之间,同时检测表明细胞内定位.

结论:

这是第一个TRA ICP-MS方法,能够在没有大量样本准备的情况下准确地分析高度介质中的单个性细胞.
该方法允许定量评估重金属生物积累和极端爱好者的纳米粒子形成.
这一进步显著扩大了TRA ICP-MS在分析具有挑战性的生物样本中的适用性.

关键词:

这种植物是Haloferax mediterranei.类微生物类微生物生物积累的生物积累矩阵效应是一种矩阵效应.在线稀释在线稀释纳米粒子是一种纳米粒子.一个单细胞的单细胞.

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