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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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使用常规显微镜使用颜色强度条形码在一轮中进行高复合空间RNA成像.

Tianyi Chang1, Shihui Zhao1,2, Kunyue Deng1,2

  • 1Biomedical Pioneering Innovation Center (BIOPIC), Peking University International Cancer Institute, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.

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通过单回合成像 (PRISM) 在现场对RNA进行分析,可以通过扩大色彩容量,使用标准显微镜进行64倍位RNA成像. 这一突破有助于在各种生物系统中进行详细的空间转录基因分析.

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 显微镜的使用方法

背景情况:

  • 传统的光显微镜将空间RNA成像限制在少数道上.
  • 顺序光 in situ 杂交需要复杂的仪器来进行多重复合.

研究的目的:

  • 介绍一种新的方法,PRISM,用于高复合空间RNA成像.
  • 使用颜色强度分级扩展RNA编码能力.
  • 通过常规显微镜实现多重RNA成像.

主要方法:

  • 开发了PRISM (通过单回合成像在现场对RNA进行剖析).
  • 利用色彩强度分级来扩大编码能力.
  • 采用半径向量过来进行代码词区分.
  • 在单个回合中实现了64倍位的彩色条形码RNA成像.

主要成果:

  • 证明了PRISM在多种组织中的多功能性.
  • 创建了一个3D小鼠胚胎发育图谱 (E12.5-E14.5).
  • 创建了一个准3D的人类肝细胞癌瘤-正常过渡景观.
  • 在小鼠大脑中绘制了3D细胞图谱和亚细胞RNA定位.
  • 确定了癌症相关纤维细胞在瘤免疫微环境中的作用.

结论:

  • PRISM显著提高了空间RNA成像能力.
  • 该方法支持使用可访问的仪器进行高度复杂的分析.
  • PRISM提供了对发育生物学,癌症和神经科学的新见解.