一个哈普洛型解析的染色体景观将cis调节变体与类植物的特征变异连接起来
Isaac A Diaz1, Talieh Ostovar2, Jinfeng Chen2
1Department of Botany and Plant Sciences, UC Riverside, Riverside, CA, USA.
BMC genomics
|October 31, 2025
概括
我们将遗传和表观遗传因素与类植物的等位基因特异性基因表达联系起来. 这揭示了DNA和染色质的变异如何影响诸如水果大小等特征,以改善作物.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 植物科学 植物科学
背景情况:
- 在cis调节序列中的遗传和表观遗传变化会改变基因表达和表型.
- 阶段性基因组组合允许详细分析异合体生物体中的基因调节.
研究的目的:
- 通过分相基因组组装来探索等位基因特异性基因表达的分子特征.
- 研究遗传变异,表观遗传状态和果中的基因表达之间的关系.
主要方法:
- 组装了一个局部分相基因组,用于一个普通杂交 ('Fairchild').
- 配对的等位基因特异性基因表达与单位基因特异性染色体状态 (可访问性,基因组修饰,DNA甲基化).
- 综合遗传和表观遗传数据与水果特征的高通量表型分析.
主要成果:
- 30%的等位基特异性表达变化与类型因子相关,主要是染色质可访问性和基因组修饰.
- 与异位基因特异性表达相关的促进体中的结构变异和DNA转位子 (hAT,MULE-MuDR).
- 在开放的染色体区域中丰富的特征相关变异;与减少水果大小相关的吉普赛逆转移子插入.
结论:
- 剖析了一种异合体果品种中遗传变异和分子表型之间的相互作用.
- 确定了对农业相关特征具有潜在功能影响的 cis-regulatory 序列.
- 通过了解基因调节,为类植物的遗传改进提供了洞察力.
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