相关实验视频
Updated: Jan 12, 2026

07:30
Ensemble Force Spectroscopy by Shear Forces
Published on: July 26, 2022
1.9K
通过声力机械力调节一个庞大的生物分子结构集.
Pravin Pokhrel1, Grinsun Sharma2, Jaren Jenyk1,3
1Department of Chemistry and Biochemistry, Kent State University, Kent, OH, 44242, USA.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|October 31, 2025
概括
低功率超声波产生声机械力,使DNA结构可逆地展开,无论是在体外还是在细胞内. 这种技术释放癌症药物,为精准医学提供了一种新的方法.
科学领域:
- 生物物理学的生物物理.
- 分子生物学分子生物学
- 生物医学工程 生物医学工程
背景情况:
- 像光学针这样的单分子技术可以操纵生物分子,但在规模和细胞应用方面是有限的.
- 现有的方法在实现高灵敏度和高效率的基于力量的分子操纵方面面临挑战,特别是在活细胞内.
研究的目的:
- 开发一种可扩展的,非侵入性的方法,用于产生声机械力来操纵生物分子结构.
- 研究低功率超声波对DNA结构可逆展开和细胞内向药物递送的潜力.
主要方法:
- 利用低功率超声波 (<5.3 mW cm−2) 来产生声力学力,对光学子进行校准.
- 应用超声波力来展开DNA结构 (G-四重复,发针) 并观察癌细胞中DNA载体的药物释放.
主要成果:
- 在5.3 mW cm-2时,至少29 pN的量化声机械力,能够同时和可逆地展开多个DNA结构.
- 证明了细胞内声机械展开,导致从DNA毛针载体释放多克索鲁比,随后有针对性的癌细胞死亡.
结论:
- 低功率超声波提供了一种灵活的,非侵入性的方法,用于操纵没有固定方向的大量生物分子.
- 这种声机械平台显示出在分子生物物理学,智能材料和精密癌症医学领域的应用潜力显著.
相关概念视频
Mechanical Protein Functions
5.5K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
5.5K
Mechanisms of Membrane-bending
3.3K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
3.3K
Cell-matrix's Response to Mechanical Forces
3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
3.4K

