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在PRRSV-2感染的巨细胞中发生线粒体功能障碍

Thien-Phong Vu Manh¹, Alba Frias-De-Diego², Abigail Williams²

¹Aix Marseille Univ, CNRS, INSERM, CIML, Marseille, France.

Frontiers in immunology

|October 31, 2025

概括

猪生殖和呼吸系统综合征病毒 (PRRSV) 感染会损害肺部巨细胞中的线粒体功能,而猪膜巨细胞 (PAMs) 显示出比肺内血管巨细胞 (PIMs) 更大的影响. 这项研究揭示了猪对PRRSV-2的菌株特异性反应.

科学领域:

兽医病毒学兽医病毒学
免疫学免疫学免疫学
细胞的新陈代谢

背景情况:

猪生殖和呼吸系统综合征病毒 (PRRSV) 在猪业中造成重大经济损失.
PRRSV感染肺部巨细胞,导致免疫反应受损.
了解PRRSV-2菌株变异及其对巨细胞功能的影响对于疾病控制至关重要.

研究的目的:

调查不同PRRSV-2菌株感染的猪膜巨细胞 (PAMs) 和肺内血管巨细胞 (PIMs) 的代谢和免疫特征.
评估PRRSV-2感染对线粒体功能和肺巨细胞中氧化应激的影响.
为了确定巨对不同PRRSV-2菌株的反应的差异.

主要方法:

主要的PAM和PIM被北卡罗来纳州 (NC) 的PRRSV-2菌株 (NC134,NC174) 和原型菌株 (VR2232) 感染 *in vitro*.
进行了转录组分析 (RNA-Seq) 和基因表达验证 (RT-qPCR,NanoString).
功能测试 (海马) 评估了线粒体呼吸,同时测量了反应性氧物种 (ROS) 和氧化 (NO) 生产.

主要成果:

PRRSV-2感染降低了参与氧化酸化和PAMs电子运输链的基因.
与未感染的细胞相比,PIMs表现出有限的转录基因变异.
PAMs在PRRSV-2感染时显示出线粒体适应性比PIMs更显著的下降,与转录组数据一致.
在受感染的巨细胞和对照巨细胞之间没有观察到ROS或NO产生的显著差异.

结论:

PRRSV-2感染显著改变了肺巨细胞,特别是PAMs中的线粒体功能.
不同的PRRSV-2菌株可以在肺巨子组中引起不同的反应.
这些发现提供了对PRRSV-2病变的洞察力,并突出了巨细胞对感染的特异性反应.

关键词:

在NanoString中使用.这是PRRSV-2病毒.巨细胞是什么?巨细胞是什么?线粒体功能障碍线粒体功能障碍猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪海马技术技术海马技术翻译学翻译学翻译学翻译学

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