在NIHL中,SOD调解了线粒体表观遗传调节
Liuwei Shi1,2, Caiping Li3, Dianpeng Wang1,3
1Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen, China.
Frontiers in cellular neuroscience
|October 31, 2025
概括
职业噪音引起的听力损失 (NIHL) 与线粒体DNA (mtDNA) D-循环低甲基化和氧化应激有关. 在NIHL病例中观察到较低的超氧化物脱酶 (SOD) 和较高的甲 (MDA),这表明存在表观遗传机制.
科学领域:
- 环境健康 环境健康
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
背景情况:
- 职业噪音暴露是导致听力损失的重要危险因素.
- 线粒体功能障碍和氧化应激与噪音引起的听力损失 (NIHL) 有关.
- 表观遗传修饰,如DNA甲基化,可能在NIHL病变发生过程中发挥作用.
研究的目的:
- 调查线粒体DNA (mtDNA) D-环区域甲基化与NIHL和没有NIHL的工人的氧化应激标记之间的关联.
- 探索mtDNA甲基化,氧化应激和听力值之间的关系.
- 为了确定NIHL的潜在表观遗传生物标志物.
主要方法:
- 一项涉及150名工人的横截面研究,分为控制,暴露于噪音和NIHL组.
- 在双硫酸盐转换后使用定量PCR来评估mtDNA D-循环甲基化.
- 实时PCR测量了线粒体DNA拷贝数 (mtDNA-CN). 实时PCR测量了线粒体DNA拷贝数.
- 氧化应激标志物 (SOD,GPX,TAS,MDA) 通过使用特定测试来定量.
主要成果:
- 与对照组相比,NIHL病例表现出较低的超氧化物脱酶 (SOD) 和较高的甲 (MDA) 水平.
- 在NIHL小组中,mtDNA D-循环甲基化水平显著降低.
- 在NIHL组中观察到较高的mtDNA复制数 (mtDNA-CN).
- D环甲基化与SOD正相关,与MDA负相关.
- 调解分析表明,SOD调解了D环甲基化和听力值之间的联系.
结论:
- 噪音诱导的氧化失衡,以减少SOD为特征,可能导致mtDNA D-循环低甲基化,导致NIHL.
- mtDNA D-循环甲基化模式和氧化应激标记可以作为NIHL的初步生物标记.
- mtDNA的表观遗传调节可能是职业听力损失发展的关键机制.
相关概念视频
Epigenetic Regulation
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Animal Mitochondrial Genetics
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
Epigenetic Regulation
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Genomic Imprinting and Inheritance
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
Mitochondria
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
Epigenetic Regulation
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...


