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Updated: Jan 12, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
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Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

Published on: November 23, 2016

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一个被取代的氨酸探针用于增强的基于过氧化酶的近邻蛋白质标签

Pornchai Kaewsapsak1,2, Nattavorapon Tantisasirat3, Sucheewin Krobthong3

  • 1Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Pathum Wan Bangkok 10330 Thailand.

RSC chemical biology
|October 31, 2025
PubMed
概括

一个新的化学探针,DBA-Me,增强了细胞中的蛋白质标记,用于研究细胞功能和疾病. 与传统的生物-探针相比,它提供了更好的效率和恢复.

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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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