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通过生物信息学分析,探索与铁亡相关的基因和途径在介酶干细胞衰老中的参与

Laleh Mavaddatiyan¹, Yasaman Khamineh¹, Leila Taghiyar²

¹Department of Animal Sciences and Marine Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

Frontiers in aging

|October 31, 2025

概括

衰老会损害介酶干细胞 (MSCs) 的功能. 这项研究确定了参与MSC衰老的关键铁亡相关基因 (FRDEG),为增强再生能力和抗衰老提供了潜在的治疗点.

科学领域:

干细胞生物学干细胞生物学
衰老的研究研究.
细胞死亡机制细胞死亡机制

背景情况:

介质细胞干细胞 (MSC) 对于组织修复至关重要,但随着年龄的增长而下降.
与年龄相关的MSC功能障碍可能会加速衰老.
铁,一种依赖于铁的细胞死亡,与衰老有关,但其在MSCs衰老中的作用尚不清楚.

研究的目的:

在老化MSC中研究与铁亡相关的基因 (FRDEGs).
确定关键的FRDEG及其监管网络.
探索MSC衰老的潜在治疗点.

主要方法:

对FRDEGs的GSE68374数据集的分析.
基因本体学 (GO) 和KEGG通路分析.
蛋白与蛋白相互作用 (PPI) 网络的构建和枢纽基因的识别.
使用独立数据集和qRT-PCR进行验证.

主要成果:

确定了131个涉及细胞衰老,铁亡和氧化应激的FRDEGs.
在数据集中验证了八个关键的FRDEG (ATF3,EZH2,SNCA,PTGS2,NOX4,CDKN2A,SQSTM1,IL6).
构建了酶,转录因子和microRNAs的综合调节网络.

结论:

特定的FRDEG在MSC衰老和铁死中发挥着关键作用.
准这些关键基因可能会改善MSC的再生能力.
这项研究提供了关于减轻与年龄相关的MSC功能障碍的见解.

关键词:

衰老的衰老衰老的衰老生物信息学是一种生物信息学.丰富分析是一种丰富分析.铁性化 (ferroptosis) 是一种介质细胞干细胞介质细胞干细胞

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