开发和初步机械分析复合泡性肝脏保护颗粒
Xinyao Liu1,2, Ruru Wang1,2, Yumeng Li1,2
1Department of Pharmacy, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China.
Frontiers in nutrition
|October 31, 2025
概括
这项研究优化了针对急性肝损伤 (ALI) 的传统中医药制剂 (CEHG). 在动物模型中,CEHG通过调节关键信号通路和减少肝损伤标志物,显示出显著的肝保护作用.
科学领域:
- 药理学 药理学是指药理学的学科.
- 肝病学 肝病学是一种肝病学.
- 传统中国医药 传统中国医药
背景情况:
- 急性肝损伤 (ALI) 是一个重要的全球健康问题,原因多种多样.
- 传统中医 (TCM) 由于其低毒性和广泛的生物活性,提供了潜在的治疗策略.
- 虽然单个TCM成分显示肝脏保护,但对ALI的复合制剂的疗效不太清楚.
研究的目的:
- 为了优化提取和颗粒形成的化合物草药来保护肝脏 (CEHG).
- 通过网络药理学和体内研究,阐明CEHG的肝保护机制.
- 为CEHG在治疗急性肝损伤中的临床应用提供科学验证.
主要方法:
- 使用直角实验,AHP-权计分和验证测试,优化了水提取和颗粒形成过程.
- 采用网络药理学来预测和识别参与CEHG行动的关键信号通路.
- 使用碳四化物 (CCl4) 诱导的急性肝损伤小鼠模型来评估CEHG的治疗效果和机制.
主要成果:
- 经过验证的可靠的提取和颗粒形成过程,CEHG具有一致的活性成分水平 (阿尔比佛林,帕埃尼佛林,液化素,甘酸,甘酸A).
- 网络药理学发现HIF-1,p53和FoxO信号通路对于CEHG的肝保护作用至关重要.
- 在受伤的老鼠中,CEHG的使用显著降低了肝酶 (AST,ALT),炎症性细胞因子 (IL-6,IL-1β,TNF-α),氧化应激标志物 (MDA,ROS) 和与亡相关的蛋白质 (P53,Bax,HIF-1α),同时提高了抗氧化能力 (SOD,GSH-Px) 和生存蛋白质 (Bcl-2).
结论:
- 成功优化了CEHG制剂,并证明了它对大鼠中CCl4诱导的ALI的显著肝保护作用.
- 阐明了CEHG的机制,包括调节HIF-1,p53和FoxO通路,以及抗炎和抗氧化作用.
- 这些发现为进一步开发和临床使用CEHG治疗急性肝损伤提供了科学基础.
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