小RNAs通过调节Rho利用位点可访问性来积极和消极地控制转录延长
Kristen R Farley1, Andrew K Buechler1, Colleen M Bianco1
1Department of Microbiology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
大肠杆菌中的小RNA (sRNA) 通过控制转录终结来调节基因表达. 这些sRNAs调节cfa mRNA 5'未翻译区域的Rho-依赖终结,影响转录水平的基因表达.
科学领域:
- 细菌的基因调节 细菌的基因调节
- 分子生物学分子生物学
- RNA生物学的RNA生物学
背景情况:
- 细菌采用多层调节策略来快速调节基因表达.
- 小RNAs (sRNAs) 是mRNA转化和稳定性的关键转录后调节者.
- 以前已经证明,大肠杆菌中的多个sRNAs (RydC,CpxQ,ArrS,GcvB,OxyS) 能够调节环胺脂肪酸合成酶 (cfa) 的mRNA.
研究的目的:
- 研究sRNAs在调节cfa mRNA转录延长中的作用.
- 确定sRNA调节Rho-依赖转录终结的机制.
- 为了阐明cfa 5' UTR内sRNA结合位如何影响终结.
主要方法:
- 在cfa 5' UTR中对Rho-依赖转录终结的分析.
- 研究sRNA结合对终止效率的影响.
- 对cfa 5' UTR和sRNA相互作用部位的结构分析.
- 评估sRNAs对RNase E介导的mRNA周转的影响.
主要成果:
- 在cfa 5' UTR中富含甲胺的区域对于过早的Rho-依赖终止至关重要.
- 对cfa表达的sRNA依赖调节取决于Rho.
- 激活的sRNA RydC对RNase E介导的周转率的影响很小.
- 抑制的sRNACpxQ增强了Rho依赖终止,而激活的sRNARydC则减少了它.
结论:
- sRNAs可以通过调节转录延长来调节转录水平上的基因表达.
- sRNA利用多功能机制来控制基因表达,包括影响Rho-依赖终结.
- 长5' UTRs为涉及sRNA和转录延长控制的复杂调节机制提供平台.
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