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在Staphylococcus分离物中检测万科米辛和提科普拉宁耐药性:哪种方法更可靠?

Ruveyda Alacahan-Durer^1,2, Güner Söyledir³

¹1Department of Medical Microbiology, Faculty of Medicine, University of Health Sciences, Istanbul, Türkiye.

Acta microbiologica et immunologica Hungarica

|October 31, 2025

概括

自动化系统可能会高估Staphylococcus aureus和CoNS中的糖抗生素耐药性. 汁微稀释对于确认万科米辛和提科普拉宁的敏感性至关重要,尤其是在怀疑耐药性时.

科学领域:

临床微生物学临床微生物学
抗微生物耐药性抗微生物耐药性
传染性疾病传染性疾病

背景情况:

自动化系统在临床实验室中普遍用于抗生素敏感性测试.
这些系统可能不准确地检测到Staphylococcus aureus和CoNS中的糖抗性.
精确检测糖抗性对于有效治疗至关重要.

研究的目的:

评估实验室方法检测糖抗性的可靠性.
为了比较自动化系统,梯度扩散和微稀释 (BMD) 对于万科米辛和提科普拉宁.
评估自动化系统在识别抗胰岛素细菌方面的准确性.

主要方法:

对87个临床分离物 (S. aureus和CoNS) 的回顾性分析,最初标记为耐药.
使用自动化系统,梯度扩散和参考BMD方法测试隔离物.
基于欧洲抗菌素敏感性测试委员会 (EUCAST) 断点的结果解释.

主要成果:

所有的分离物都是通过BMD对万科米辛和提科普拉宁敏感的.
自动化系统显示,万科米的一致性为100%,而提科普拉宁的一致性仅为77%.
五个S. aureus分离体通过自动化/梯度方法显示了teicoplanin耐药性,但对BMD敏感.

结论:

自动化系统可能会高估葡萄糖抗性,特别是对于teicoplanin.
在怀疑糖抗性时,乳汁微稀释 (BMD) 对于确认测试至关重要.
准确的敏感性测试可以防止错误分类,并确保适当的患者管理.

关键词:

抗微生物耐药性抗微生物耐药性对抗微生物的敏感性测试.牛微稀释的方法泰科普拉尼尼泰科普拉尼尼这就是Vancomycin.

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