对于非结核性菌根菌的MALDI-TOFMS的诊断性能:系统性审查和元分析
Xiaodan Wang1, Lisha Zhao1, Huihan Zhao2
1Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
概括
矩阵辅助激光脱/离子化飞行时间质谱法 (MALDI-TOF MS) 可以准确识别大多数非结核菌株 (NTM). 虽然对快速生长的物种有效,但在区分密切相关或罕见的NTM方面仍然存在挑战.
科学领域:
- 临床微生物学 临床微生物学
- 诊断技术 诊断技术 诊断技术
- 传染性疾病 传染性疾病
背景情况:
- 非结核菌 (NTM) 构成了日益增长的临床挑战.
- 准确识别NTM对于有效治疗至关重要.
- 矩阵辅助激光消耗/电离飞行时间质谱 (MALDI-TOF MS) 提供了快速NTM识别的潜力.
研究的目的:
- 系统地评估MALDI-TOF MS用于NTM识别的诊断准确性.
- 评估影响MALDI-TOF MS性能在NTM检测中的因素.
- 在临床微生物学中提供基于证据的MALDI-TOF MS实用性的概述.
主要方法:
- 2011年至2024年5月期间发表的研究的系统审查和元分析.
- 包括对MALDI-TOF MS与NTM识别参考标准进行比较的研究.
- 使用随机效应模型和子组分析计算聚合识别率.
主要成果:
- 在临床NTM分离物中,MALDI-TOF MS显示了88%的整体识别准确度.
- 快速生长的真菌菌 (93%) 与缓慢生长的真菌菌 (89%) 相比,观察到更高的准确性.
- 固体介质培养比液体或共同培养系统产生更好的结果 (91%).
结论:
- MALDI-TOF MS是一种非常准确的工具,用于识别具有临床意义的NTM,特别是快速生长的物种.
- 区分密切相关或罕见的NTM物种仍然是一个局限性.
- 为了改善NTM诊断,需要进一步优化和标准化MALDI-TOF MS.
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