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颗粒对骨细胞在骨重塑中的功能的影响:一个体外研究

Neibal Almabrok¹, K G Aghila Rani², Waad Kheder³

¹Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, United Arab Emirates.

|October 31, 2025

概括

二氧化 (TiO2) 微粒 (MP) 和纳米粒子 (NP) 对骨细胞功能产生负面影响. TiO2 MPs比NP更严重地破坏骨细胞功能,抑制骨形成并促进骨再吸收.

科学领域:

生物材料科学生物材料科学
细胞生物学细胞生物学
整形外科整形外科整形外科

背景情况:

骨重塑涉及骨质细胞调节骨质细胞和骨质细胞.
二氧化 (TiO2) 植入物可以浸出颗粒,引发了对骨细胞功能的担忧.

研究的目的:

研究TiO2微粒 (TiO2-MPs) 和纳米粒子 (TiO2-NPs) 对骨细胞功能的影响.
评估TiO2颗粒对骨质母细胞生成和骨质母细胞生成的影响.

主要方法:

用TiO2-MPs或TiO2-NPs治疗的MLO-Y4骨质细胞样细胞.
条件介质 (CM) 用于间接的骨质细胞-骨质细胞和骨质细胞-骨质细胞共同培养.
测定了胆固醇释放,基因表达 (RANKL,OPG,TNF-α,IL-1β,TRAP,Cat K) 和矿化情况.

主要成果:

与TiO2-NPs相比,TiO2-MPs显著增加了硬质素,RANKL,TNF-α,IL-1β,TRAP和Cat K的表达.
TiO2-MPs抑制了骨质细胞分化标志物 (ALP,OPG) 和矿化.
TiO2-NP对这些标志物的影响较小.

结论:

TiO2颗粒,特别是微粒,破坏骨细胞的功能.
泄的TiO2颗粒抑制骨质母细胞发生,并通过硬质素和RANKL促进骨质母细胞发生.
微粒子暴露对骨重塑的风险比纳米粒子暴露更大.

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