从血液中分离出的Lacticaseibacillus rhamnosus菌株的基因组特征
Piotr Jarocki1, Jan Sadurski1, Martyna Siuda1
1Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, Lublin, Poland.
PloS one
|October 31, 2025
概括
对Lacticaseibacillus rhamnosus血液分离物的基因组分析揭示了显著的多样性. 这些发现强调了需要仔细选菌株来使用益生菌,因为益生菌和致病基因重叠.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 益生菌 益生菌 益生菌
背景情况:
- 乳酸菌 (Lacticaseibacillus rhamnosus) 在食品和补充剂中被认为具有健康益处.
- 罕见的不良反应,主要是免疫功能受损的个体,需要进一步调查.
- 了解L. rhamnosus菌株的基因组基础对于安全性和有效性至关重要.
研究的目的:
- 从血液中分离出来的七种L. rhamnosus菌株的基因组测序和表征.
- 将这些分离物与来自不同来源的多种L. rhamnosus菌株进行比较.
- 确定与促进健康的特征和潜在的致病性相关的遗传决定因素.
主要方法:
- 使用Illumina和牛津纳米孔技术进行混合基因组测序.
- 用公开可用的L. rhamnosus基因组进行比较基因组分析.
- 核心基因组多部位序列类型化 (cgMLST),先导和CRISPR模块分析.
- 基因分析以确定功能决定因素.
主要成果:
- 对于七个血液分离物,获得了完整的基因组 (2.963.13 Mb,GC含量为46.7-46.8%).
- 隔离物显示出与来自不同环境的菌株的遗传关系,包括临床样本.
- 通过cgMLST或prophage/CRISPR分析,没有发现与益生菌菌株L. rhamnosus GG的相似性.
- 确定了与细菌素生产,粘附,健康益处和致病性相关的基因.
- 观察到与益生菌功能和已知的毒性因子相关的基因之间存在显著的重叠.
结论:
- L. rhamnosus 的血液分离物表现出相当大的基因组多样性.
- 某些遗传决定因素具有双重作用,可能会对益生菌效应和致病性产生影响.
- 菌株级基因组评估对于安全有效地选择L. rhamnosus用于益生菌应用是必不可少的.
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