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秋季晚些时候开卷红的原因是BoCBF2/BoMYB2协同调节BoDFR表达的作用

Xinyuan Li¹, Zhichao Gong¹, Guoliang Yang¹

¹College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.

Plant physiology

|October 31, 2025

概括

装饰菜叶在寒冷的时候会变红,这是因为它含有安东素. 研究人员发现BoCBF2/BoMYB2模块调节BoDFR基因,控制这种颜色变化,并提供植物对低温反应的见解.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
生物化学生物化学

背景情况:

在寒冷的条件下,由于安托素的积累,装饰菜会呈现出红色的叶子.
精确的分子机制驱动低温诱导的红在开罗仍然在很大程度上是未知的.

研究的目的:

在寒冷压力下,阐明负责花菜中安东素合成和叶子红化的分子途径.
确定关键的调节基因和转录因子,涉及到植物对低温的反应.

主要方法:

基于地图的克隆被用来识别安托西亚宁合成基因BoDFR.
用RNA测序 (RNA-seq) 和cis作用元素分析来预测上游调节因素.
分子测定包括酵母单杂交,电泳性移动性转移,GUS和光酶记者测定验证了基因相互作用和转录调节.

主要成果:

鉴定出BoDFR基因对于氨酸合成至关重要;其沉默减少了色彩.
BoMYB2被确定为一种上游转录因子,它与BoDFR促进体结合,激活其表达.
发现了一种调节模块BoCBF2/BoMYB2,其中BoCBF2与BoMYB2相互作用,在低温下协同调节BoDFR表达.

结论:

这项研究揭示了一种涉及BoCBF2,BoMYB2和BoDFR的新型调节途径,用于控制装饰性胡卜中的安托西亚尼积累和叶子红色.
这为植物在应对寒冷压力时的颜色变化提供了分子理解,为增强装饰性特征提供了潜力.

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