通过通过MdCOMT9增强红素生物合成途径来减轻苗中的毒性
Lei Fang1, Xinyi Zhou2, Jiale Chen2
1Guizhou Engineering Research Center for Fruit Crops, College of Agriculture, Guizhou University, Guiyang, Guizhou Province 550025, China; College of Agriculture, Guizhou University, Guiyang 550025, China.
Journal of hazardous materials
|October 31, 2025
概括
(Si) 应用通过增强素合成来减轻 (Al) 在果苗中的毒性. 过度表达MdCOMT9基因显著增加了素,改善了植物的根生长和Al耐受性.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- (Al) 的毒性严重限制了果的生长和产量.
- 已知 (Si) 通过减少金属吸收和氧化损伤,减少重金属对植物的影响.
研究的目的:
- 调查 (Si) 在减轻 (Al) 压力中的作用.
- 阐明基底的分子机制Si-介导的Al耐受性.
主要方法:
- 在 Al 压力下对果幼苗施加外源Si.
- 转录组和代谢组分析以确定关键的代谢途径和基因.
- 在果植物中过度表达MdCOMT9基因,以研究其在Al应激反应中的功能.
- 测量Al度,素含量,根形态和酶活动.
主要成果:
- 外源Si的应用降低了果植物中的Al度,缓解了Al诱导的压力,并促进了苗木的生长.
- Si主要通过烯生物合成,酸代谢和黄生物合成来减轻Al的毒性,而烯生物合成至关重要.
- 素合成基因 (CYP98A,COMT,HCT,4CL) 的升级增强了素合成和Al与细胞壁的结合.
- 过度表达MdCOMT9的植物在Al压力下显著增加了红素含量 (95.8%),改善了根生长 (40.5%的长度增加),并增强了抗氧化酶活动.
结论:
- 外源Si有效地减轻了果幼苗在分子层面上的Al压力.
- 基因MdCOMT9通过增强氨酸合成,在氨酸应激反应中发挥关键作用.
- 这项研究为了解果Al耐受机制和培育Al耐药品种提供了理论基础.
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