相关实验视频

Updated: Jun 29, 2026

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
07:41

Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography

Published on: October 17, 2018

9.3K

从微/纳米颗粒中通过薄膜嵌提取TEM片加强了FIB方法

Bo Deng1, Li Lei1, Lei Wang2

  • 1Advanced Material Analysis and Test Center, Xi'an University of Technology, Xi'an, Shaanxi 710048, China; School of Materials Science and Engineering, Xi'an University of Technology, Xi'an, Shaanxi 710048, China.

Micron (Oxford, England : 1993)
|October 31, 2025
PubMed
概括

一种新的薄膜嵌入强化聚焦离子束 (FES-FIB) 技术精确地提取了超薄层,用于显微镜. 这种方法克服了传统嵌入的问题,改善了分析微/纳米粒子的样本准备.

关键词:
聚焦的离子束.纳米/微型结构在TEM的样本准备过程中.薄膜嵌入式嵌入式薄膜嵌入式

更多相关视频

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments08:31

Sample Preparation and Experimental Design for In Situ Multi-Beam Transmission Electron Microscopy Irradiation Experiments

Published on: June 27, 2022

2.2K
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography07:00

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Published on: August 5, 2021

4.0K

相关概念视频

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...