微生物通过一种新的β-氨酸路径产生酸
Da-Hee Ahn1, Yoo-Sung Ko2, Cindy Pricilia Surya Prabowo2
1Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Four Program), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
Metabolic engineering
|October 31, 2025
概括
研究人员开发了一种新的基于生物的方法,使用工程细菌生产酸. 这种新的途径,通过β-氨酸,在大肠杆菌和Corynebacterium glutamicum中实现了高产量,提供了一个可持续的替代方案.
科学领域:
- 生物技术和合成生物学
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- 酸是一种重要的平台化学物质,具有多种工业应用.
- 使用Propionibacterium物种的传统微生物生产方法受到缓慢生长和基因工程挑战的限制.
- 开发高效和可持续的生物基生产途径对于工业可行性至关重要.
研究的目的:
- 设计一种新的生物合成途径,利用β-氨酸路径从葡萄糖中产生酸.
- 优化大肠杆菌和Corynebacterium glutamicum中的工程菌株,以生产高位酸.
- 建立一种不依赖于维生素B12的可持续替代传统酸制造.
主要方法:
- 设计了一条由两个模块组成的生物合成途径:上游β-alanine形成和下游propionic acid形成.
- 通过酶查和流量优化 (PPC) 验证和优化了Escherichia coli W3110中的途径.
- 转移并进一步设计了Corynebacterium glutamicumATCC 13032中的途径,包括途径中断 (ack-pta) 和消灭代谢途径 (prpD2B2C2).
主要成果:
- 在工程化大肠杆菌中通过食批发发酵实现了14.8 g/L的酸.
- 工程Corynebacterium glutamicum产生了47.4g/L酸,这是异质生产的创纪录.
- 展示了一种新的,不依赖维生素B12的生物生产策略.
结论:
- 开发的β-氨酸路径为生物基酸生产提供了一种高效且可扩展的方法.
- 由于其耐酸性,Corynebacterium glutamicum 作为高位酸合成的优质宿主.
- 这项工作为传统酸生产方法提供了一种可持续和工业相关的替代方案.
相关概念视频
Microbial Fermentation
1.3K
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
1.3K
Amino Acid Catabolism
966
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
966
Alkylation of β-Diester Enolates: Malonic Ester Synthesis
4.1K
Malonic ester synthesis is a method to obtain α substituted carboxylic acids from ꞵ-diesters such as diethyl malonate and alkyl halides.
4.1K
Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis
4.4K
Acetoacetic ester synthesis is a method to obtain ketones from alkyl halides and β-keto esters. The reaction occurs in the presence of an alkoxide base that abstracts the acidic proton of the β-keto esters. The step results in an enolate ion which is doubly stabilized. The enolate then reacts with an alkyl halide via the SN2 process to produce an alkylated ester intermediate with a new C–C bond. The hydrolysis of the intermediate, followed by acidification, results in an...
4.4K
Amino Acid Biosynthetic Pathways
1.0K
Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
1.0K
Fates of Pyruvate
10.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
10.4K


